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丁香实验推荐阅读
Evolution of Electroporated DNA Vaccines

Vaccines have evolved for hundreds of years, but all utilize the premise that safely pre-exposing the host to some component of a pathogen allows for enhanced immune recognition, and potential protection from disease, upon encountering the pathogen at a later date. Early vaccination stra ...

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Low-Energy DC Current Ablation in a Mouse Tumor Model

Irreversible electroporation has recently been applied to tissue and tumor ablation. This animal model was developed to evaluate optimal parameters for subcutaneous tumor ablation. The immortalized Panc1 tumor cell line was used for generating tumors via subcutaneous injecti ...

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Electrochemotherapy in Veterinary Oncology: From Rescue to First Line Therapy

Electroporation is a delivery technique that is gaining popularity among the veterinary community due to its low cost, ease of application, and flexibility. It combines the administration of pharmaceutical compounds such as chemotherapy agents, antisense, and plasmids to the appl ...

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Managing Local Swelling Following Intratumoral Electro-Chemo-Gene Therapy

Delivering genes and other materials directly into the tumor tissue causes specifically localized and powerfully enhanced efficacy of treatments; however, these specific effects can cause rapid, drastic changes in the appearance, texture, and consistency of the tumor. These chan ...

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In Vivo Electroporation-Mediated Gene Delivery to the Beating Heart

Gene therapy may represent a promising alternative strategy for cardiac muscle regeneration. In vivo electroporation, a physical method of gene transfer, has recently evolved as an efficient method for gene transfer. Here, we describe two protocols involving in vivo electroporati ...

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Electroporation-Mediated Delivery of Genes in Rodent Models of Lung Contusion

Several of the biological processes involved in the pathogenesis of acute lung injury and acute respiratory distress syndrome after lung contusion are regulated at a genetic and epigenetic level. Thus, strategies to manipulate gene expression in this context are highly desirable not o ...

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Delivery of Plasmid DNA into Dental Tissues of Developing Rat Teeth by Electroporation

Gene transfer by electroporation is a versatile technique that can effectively deliver DNA or RNA into almost all types of cells or tissues. As a widely used nonviral approach, electroporation possesses several advantages over viral methods including non-immunogenicity and local t ...

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DNA Delivery in Adult Mouse Eyes: An Update with Corneal Outcomes

Ocular injection (intravitreal, subretinal, or into the anterior space) is an efficient approach to deliver many classes of drugs, cells, and other treatments to various cell types of the eye. In particular, subretinal injection is efficient since delivered agents accumulate as there is no ...

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Delivery of DNA into the Central Nervous System via Electroporation

Electroporation of non-viral plasmid DNA is a valuable tool to alter gene expression in the adult central nervous system. It offers a number of advantages over viral gene delivery as non-viral plasmids can integrate larger inserts and reduce the risk of inducing unintended immunological r ...

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Electroporation of siRNA into Mouse Bone Marrow-Derived Macrophages and Dendritic Cells

Dendritic cells (DC) and macrophages (MΦ) play a pivotal role in antimicrobial defense, in the regulation of immune responses, and in maintaining tissue homeostasis. The analysis of DC and MΦ function relies on primary cells albeit these cells are known to be difficult to transfect. This makes the ...

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Continuous Cell Electroporation for Efficient DNA and siRNA Delivery Based on Laminar Microfluidic Chips

Electroporation is a high-efficiency and low-toxicity physical gene transfer method. Traditional electroporation is limited to only low volume cell samples. Here we present a continuous cell electroporation method based on commonly used microfluidic chip fabrication techn ...

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siRNA Delivery via Electropulsation: A Review of the Basic Processes

Due to their capacity for inducing strong and sequence specific gene silencing in cells, small interfering RNAs (siRNAs) are now recognized not only as powerful experimental tools for basic research in Molecular biology but with promising potentials in therapeutic development. Del ...

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Intradermal Electroporation of RNA

This chapter describes the in vivo delivery of conventional mRNA or alphaviral replicon RNA via intradermal electroporation. The use of RNA in clinical applications has several potential advantages compared to DNA. For instance, RNA cannot integrate into the host genome, and it does not co ...

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Substrate-Mediated, High-Efficiency siRNA Electroporation

Here, we outline a method for surface-mediated transfection of small interfering RNA (siRNA) using electroporation. Cells are cultured on an electrode carrying siRNA. After the cells are adhered on the electrode surface, an electric pulse is applied to release siRNA from the surface and tra ...

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Electroporation-Mediated siRNA Delivery into Tumors

Electroporation-mediated gene transfer (electro-transfection) is a powerful tool to introduce nucleic acid compounds such as plasmid DNAs, antisense oligonucleotides, and short interfering RNAs (siRNAs) into the cells. Electro-transfection is a physical gene transfer me ...

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Direct Imaging of siRNA Electrotransfer at the Single-Cell Level

Short interfering RNAs (siRNAs) represent new potential therapeutic tools owing to their capacity to induce strong, sequence-specific, gene silencing in cells. Electropulsation is one of the physical methods successfully used to transfer siRNA into living cells in vitro and in vivo. A ...

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Gold Nanoparticle-Enhanced Electroporation for Leukemia Cell Transfection

Electroporation serves as an attractive nonviral gene delivery approach for its effectiveness, operational simplicity, and no restrictions of probe or cell type. The commercial electroporation systems have been widely adopted in research and clinics with protocols usually co ...

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Short-Fragment DNA-Mediated In Vivo DNA Electroporation Delivery

Electroporation is an effective physical delivery method. A variety of factors have been shown to affect the electroporation-mediated gene delivery efficiency. Here we report the usefulness of noncoding short-fragment DNA (sf-DNA) for facilitating electroporation-media ...

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Electroporation Formulation for Cell Therapy

Cell transfection efficiency often determines the success of cell-based gene therapy. Cell transfection via Nucleofector technology yields high transfection efficiency and low cytotoxicity. However, owing to trade secrecy, the components in each buffer are unknown, which not o ...

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The Impact of Non-electrical Factors on Electrical Gene Transfer

Electrical pulses directly and effectively boost both in vitro and in vivo gene transfer, but this process is greatly affected by non-electrical factors that exist during electroporation. These factors include, but are not limited to, the types of cells or tissues used, property of DNA, DNA for ...

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