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丁香实验推荐阅读
Image-Based High-Throughput Screening for Inhibitors of Angiogenesis

Automated multicolor fluorescence microscopy facilitates high-throughput quantitation of cellular parameters of complex, organotypic systems. In vitro co-cultured vascular cells form capillary-like networks that model facets of angiogenesis, making it an attracti ...

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Light Sheet Microscopy in Cell Biology

Light sheet-based fluorescence microscopy (LSFM) is emerging as a powerful imaging technique for the life sciences. LSFM provides an exceptionally high imaging speed, high signal-to-noise ratio, low level of photo-bleaching, and good optical penetration depth. This unique combin ...

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Imaging Mass Spectrometry: Viewing the Future

Imaging mass spectrometry (IMS) technology is an effective tool that is able to assess complex molecular mixtures in cells, tissues, or other sample types with high chemical specificity, allowing concurrent analysis of a variety of molecular species in a wide mass range, from small metabol ...

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A Mass Spectrometry Primer for Mass Spectrometry Imaging

Mass spectrometry imaging (MSI), a rapidly growing subfield of chemical imaging, employs mass spectrometry (MS) technologies to create single- and multi-dimensional localization maps for a variety of atoms and molecules. Complimentary to other imaging approaches, MSI provides h ...

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Imaging of Metals, Metalloids, and Non-metals by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) in Biological Tissues

The determination of the localization and distribution of essential and beneficial metals (e.g., Cu, Fe, Zn, Mn, Co, Ti, Al, Ca, K, Na, Cr and others), toxic metals (like Cd, Pb, Hg, U), metalloids (e.g., As, Se, Sb), and non-metals (such as C, S, P, Cl, I) in biological tissues is a challenging task for life science studies. Over the ...

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Matrix-Assisted Laser Desorption/Ionization and Nanoparticle-Based Imaging Mass Spectrometry for Small Metabolites: A Practical Protocol

Matrix-assisted laser desorption/ionization (MALDI)-imaging mass spectrometry (IMS, also referred to as mass spectrometry imaging ) enables visualization of the distribution of biomolecules with varied and vast structures in tissue sections. This emerging imaging techn ...

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Cellular Imaging Using Matrix-Enhanced and Metal-Assisted SIMS

Imaging mass spectrometry (IMS) allows the direct investigation of both the identity and the spatial distribution of the entire molecular content directly in tissue sections, single cells, and many other biological surfaces. We describe here the steps required to retrieve the molecul ...

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Preparation of Single Cells for Imaging Mass Spectrometry

Characterizing the molecular contents of individual cells is critical for understanding fundamental mechanisms of biological processes. Imaging mass spectrometry (IMS) of biological systems has been steadily gaining popularity for its ability to create precise chemical i ...

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Mass Spectrometry Imaging of Small Molecules Using Matrix-Enhanced Surface-Assisted Laser Desorption/Ionization Mass Spectrometry (ME-SALDI-MS)

Surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) uses inorganic particles or porous surfaces as the energy-mediating means to promote desorption and ionization of low-mass analytes of interest. With good stability during laser ablation, SALDI su ...

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Chemical Imaging with Desorption Electrospray Ionization Mass Spectrometry

Desorption electrospray ionization mass spectrometry (DESI-MS) is a relatively new ambient surface analysis method. This technique requires little or no sample preparation prior to the actual analysis. Along with other application areas, DESI-MS is used to obtain chemical images of ...

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Tandem Mass Spectrometric Methods for Phospholipid Analysis from Brain Tissue

We describe the utility of intermediate-pressure MALDI and tandem mass spectrometry (MS/MS and MS n ) for the characterization and imaging of phospholipids in brain tissue sections. The use of both MS/MS spectra and MS/MS images allows for identification of isobaric compounds. The structu ...

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Applying Imaging ToF-SIMS and PCA in Differentiation of Tissue Types

Time-of-flight secondary ion mass spectrometry (ToF-SIMS) has proven to be an extremely powerful tool for characterizing chemical distributions within biological cells and tissues. However, differentiating biological samples, e.g., cancerous cells from their normal count ...

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The Application and Potential of Ion Mobility Mass Spectrometry in Imaging MS with a Focus on Lipids

Tissue profiling and imaging by MALDI mass spectrometry has allowed the direct analysis and localization of biomolecules in tissue. However, due to the in situ nature of this technique, the complexity of tissue, and the need for a chemical matrix in MALDI, the signal recorded can be extremely comp ...

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Lipid Detection, Identification, and Imaging Single Cells with SIMS

Time-of-flight secondary ion mass spectrometry (ToF-SIMS) can be utilized to map the distribution of various molecules on a surface with submicrometer resolution. Many of its biological applications have been in the study of membrane lipids, such as phospholipids and cholesterol. For ...

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Quantitative Imaging of Chemical Composition in Single Cells by Secondary Ion Mass Spectrometry: Cisplatin Affects Calcium Stores in Renal Epithelial

A detailed protocol for quantitative single cell mass spectrometry imaging (MSI) analysis is described in this chapter with examples of the treatment of cells with anticancer drug, cisplatin. Cisplatin, cis-diamminedichloridoplatinum ii (CDDP), is widely used for the treatment of ...

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Imaging MALDI Mass Spectrometry of Sphingolipids Using an Oscillating Capillary Nebulizer Matrix Application System

Matrix deposition is a critical step in tissue imaging by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). It greatly affects the quality of MALDI imaging, especially for the analytes (such as lipids) that may easily dissolve in the solvent used for the matrix a ...

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Laser Ablation Electrospray Ionization for Atmospheric Pressure Molecular Imaging Mass Spectrometry

Laser ablation electrospray ionization (LAESI) is a novel method for the direct imaging of biological tissues by mass spectrometry. By performing ionization in the ambient environment, this technique enables in vivo studies with potential for single-cell analysis. A unique aspect of ...

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Mapping Pharmaceuticals in Rat Brain Sections Using MALDI Imaging Mass Spectrometry

Matrix-assisted laser desorption/ionization-tandem mass spectrometric method (MALDI-MS/MS) has proven to be a reliable tool for direct measurement of the disposition of small molecules in animal tissue sections. As example, MALDI-MS/MS imaging system was employed for visuali ...

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Direct Molecular Analysis of Whole-Body Animal Tissue Sections by MALDI Imaging Mass Spectrometry

The determination of the localization of various compounds in a whole animal is valuable for many applications, including pharmaceutical absorption, distribution, metabolism, and excretion (ADME) studies and biomarker discovery. Imaging mass spectrometry is a powerful tool f ...

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MALDI Direct Analysis and Imaging of Frozen Versus FFPE Tissues: What Strategy for Which Sample

Significant advances have been made in the past decade in the field of mass spectrometry imaging with MALDI ion sources (MALDI-MSI). While MALDI-MSI has high potential in the field of biology and in the clinic, a challenge for MALDI-MSI has been to adapt itself to a greater range of sample types. In partic ...

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