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丁香实验推荐阅读
Aqueous Formulation of Adjuvant-Active Nonionic Block Copolymers

Despite the recent advances in vaccine technology, there is still a need for safe and effective adjuvants to potentiate the immune responses to subunit immunogens (1). Depending on the disease in question, the vaccine-induced protection may require either a predominantly antibody-me ...

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Poly(Methyl Methacrylate) Nanoparticles As Vaccine Adjuvants

Nanoparticles are solid particles ranging in size from 1 to 1000 nm (1 μm). They consist of macromolecular materials and can be used therapeutically or prophylactically, for example, as adjuvants in vaccines or drug carriers, in which the active principle (drug or biologically active mater ...

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Liposomes As Immunological Adjuvants and Vaccine Carriers

The need for an effective and safe immunological adjuvant or vaccine carrier that could promote appropriate immune responses to vaccines is well recognized (1,2). This is especially true for most of the subunit vaccine and synthetic peptide antigens, which are not only costly or available in s ...

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Immunopotentiating Reconstituted Influenza Virosomes (IRIVs)

Immunization is the most effective defense mechanism against microbial infections today. Although highly effective vaccines are currently available for a number of infectious diseases, vaccine formulations can still be improved in a number of important areas. Issues of safety, st ...

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Mammalian Cell Display for Antibody Engineering

Antibody engineering has generally been carried out by displaying mouse or human antibodies or antibody fragments on the surface of microorganisms (phage, bacteria, and yeast). We have shown that mammalian cells can be used to display single-chain antibody fragments (scFvs) for affini ...

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Improving Antibody Binding Affinity and Specificity for Therapeutic Development

Affinity maturation is an important part of the therapeutic antibody development process as in vivo activity often requires high binding affinity. Here, we describe a targeted approach for affinity improvement of therapeutic antibodies. Sets of CDR residues that are solvent access ...

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Construction and Production of an IgG-Like Tetravalent Bispecific Antibody for Enhanced Therapeutic Efficacy

In past years, both laboratory and early clinical studies have demonstrated that bispecific antibodies (BsAbs) may have significant potential application in cancer therapy either by targeting tumor cells with cytotoxic agents including effector cells, radionuclides, drugs, ...

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Anti-CD22 Onconase: Preparation and Characterization

Antibodies can be conjugated to effector molecules to derive targeted therapeutics with properties such as cell-specific cytotoxicity. The murine anti-CD22 antibody RFB4 linked to a member of the ribonuclease A superfamily, Onconase (Onc), becomes a potential drug candidate for no ...

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Deimmunization of Monoclonal Antibodies

Immunogenicity is a major limitation to therapy with certain monoclonal antibodies and proteins. A major driver for immunogenicity is the presence of human T-cell epitopes within the protein sequence which can activate helper T-cells resulting in the sustained production of antibo ...

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AntibodyCytotoxic Agent Conjugates: Preparation and Characterization

Conjugates of antibodies with cytotoxic agents offer a targeted therapeutic strategy against cancer cells expressing target antigens. Several antibodies against various cancer cell-surface antigens have been conjugated with different cytotoxic agents that inhibit ess ...

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High-Level Production of a Humanized ImmunoRNase Fusion Protein from Stably Transfected Myeloma Cells

ImmunoRNases represent a highly attractive alternative to conventional immunotoxins for cancer therapy. Quantitative production of immunoRNases in appropriate expression systems, however, remains a major challenge for further clinical development of these novel com ...

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Antibody Fragment Expression and Purification

Interest in the potential of monoclonal antibodies (mAbs) to serve as therapeutic agents has surged in the past decade with a major emphasis on human viral diseases. There has been much attention in this area directed towards the human immunodeficiency virus type-1 (HIV-1) and promising rese ...

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Antibody Production by the Gram-Positive Bacterium Bacillus megaterium

The increasing demand for recombinant antibodies as detection reagents in research, diagnostics, and therapy requires appropriate production systems. In contrast to antibody therapies, small recombinant antibody fragments like Fab and scFv are sufficient for most applicat ...

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Scaling-Up and Production of Therapeutic Antibodies for Preclinical Studies

Monoclonal antibodies are increasingly used for treatment of various diseases, mostly cancer therapy and auto-immune diseases. New chimeric, humanized, or fully-human antibodies specific to novel or already validated targets are being developed in many academic labs as well as in co ...

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Dynamics of Antibody Domains Studied by Solution NMR

Information on local dynamics of antibodies is important to evaluate stability, to rationally design variants, and to clarify conformational disorders at the epitope binding sites. Such information may also be useful for improved understanding of antigen recognition. NMR can be used ...

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Neutralization of HIV by Antibodies

Antibodies can neutralize HIV-1 with potency and cross-reactivity that varies widely and is related but not correlated to their antigen-binding affinity. Therefore, in addition to measuring binding affinity, an evaluation of the antibody neutralizing activity in tissue cultures ...

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In Vivo Method for Establishing Synergy Between Antibodies to Epidermal Growth Factor Receptor and Vascular Endothelial Growth Factor Receptor-2

Targeted therapy for cancer is shifting towards an approach of inhibiting multiple pathways, justified in part by the ability of cancer cells to overcome the inhibition of a single pathway. However the literature is replete with preclinical data supporting the anticancer potential of nu ...

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Organizing, Exploring, and Analyzing Antibody Sequence Data: The Case for Relational-Database Managers

Technological advances in the acquisition of DNA and protein sequence information and the resulting onrush of data can quickly overwhelm the scientist unprepared for the volume of information that must be evaluated and carefully dissected to discover its significance. Few laborat ...

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Passive Immunization with Human Neutralizing Monoclonal Antibodies Against HIV-1 in Macaque Models: Experimental Approaches

After more than 20 years of intense research, a safe and effective vaccine against HIV-1/AIDS has not been developed. Passive immunization has been used as a tool to demonstrate the role of neutralizing antibodies in conferring protection against HIV-1 challenge in chimpanzees. Because th ...

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Functional Assays for Complement Regulators

As described in Chapter 1, the complement system comprises a battery of at least 20 components capable of immediate response to a foreign organism or cell, resulting in lysis or opsonization and phagocytosis. Uncontroled complement activation would result in damage to host tissues, or in ext ...

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