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丁香实验推荐阅读
Determination of Acyl Homoserine Lactone and Tetramic Acid Concentrations in Biological Samples

Within environmental communities, there is a constant struggle for survival, as nutrients are often limited. In response, bacteria have developed elaborate methods to deal with competitors. One such mechanism is the coordination of behaviors and function via the exchange of small che ...

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Detection of Bacterial Signaling Molecules in Liquid or Gaseous Environments

The detection of bacterial signaling molecules in liquid or gaseous environments has been occurring in nature for billions of years. More recently, man-made materials and systems has also allowed for the detection of small molecules in liquid or gaseous environments. This chapter will ou ...

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Detection of Autoinducer (AI-2)-Like Activity in Food Samples

The contamination, survival, and possible foodborne disease outbreaks are major issues confronting the food industry. However, from a microbial perspective, any food whether natural or processed is just another environmental niche that is available for colonization. Quorum sen ...

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Liquid Chromatography/Mass Spectrometry for the Detection and Quantification of N-Acyl-l-Homoserine Lactones and 4-Hydroxy-2-Alkylquinolines

High-performance liquid chromatography (HPLC) coupled in-line with mass spectrometry (MS) permits rapid and specific identification and quantification of N-acyl-l-homoserine lactones (AHLs) and 4-hydroxy-2-alkylquinolines (HAQs). We are presenting here methods for t ...

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Isolation of agr Quorum Sensing Autoinducers

Autoregulation of genes is often associated with quorum sensing systems where bacteria produce and secrete molecules that allow the cells to communicate with one another, leading to the activation of certain genes at certain population densities. Here we describe the identification ...

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FRET-Based Biosensors for the Detection and Quantification of AI-2 Class of Quorum Sensing Compounds

Intercellular small molecular weight signaling molecules modulate a variety of biological functions in bacteria. One of the more complex behaviors mediated by intercellular signaling molecules is the suite of activities regulated by quorum sensing molecules. These molecules ...

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Modulation of Mammalian Cell Processes by Bacterial Quorum Sensing Molecules

Microbial pathogens use a wide repertoire of pathogen-associated molecular patterns (PAMPs) that affect host cell responses through activation of intracellular signaling events in a PAMP-specific manner. Here we describe a set of western blot-based methodologies for the evalu ...

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Imaging N-Acyl Homoserine Lactone Quorum Sensing In Vivo

In order to study N-acyl homoserine lactone (AHL)-based quorum sensing in vivo, we present a protocol using an Escherichia coli strain equipped with a luxR-based monitor system, which in the presence of exogenous AHL molecules expresses a green fluorescent protein (GFP). Lungs from mice chal ...

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Defining the Structure and Function of Acyl-Homoserine Lactone Autoinducers

Quorum sensing plays a central role in regulating many community-derived symbiotic and pathogenic relationships of bacteria, and as such has attracted much attention in recent years. Acyl-homoserine lactones (AHLs) are important signaling molecules in the quorum sensing gene-r ...

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Global Expression Analysis of Quorum-Sensing Controlled Genes

Transcript profiling has improved our understanding of quorum-sensing gene regulation on a global scale. Here, we describe methods for high-quality sample preparation for use in DNA microarray and real-time PCR experiments.

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Small RNA Target Genes and Regulatory Connections in the Vibrio cholerae Quorum Sensing System

The two-component quorum sensing (QS) system, first described in the marine bacterium Vibrio harveyi and evolutionarily conserved among members of the genus Vibrio, has been best studied in the human pathogen Vibrio cholerae (1, 2). In the V. cholerae QS system, the response to the accumulation ...

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Linking Quorum Sensing Regulation and Biofilm Formation by Candida albicans

Candida albicans biofilms are surface-associated, structured communities composed of yeast, hyphal, and pseudohyphal cells surrounded by an extracellular matrix. C. albicans biofilms often lead to life-threatening systemic infections and are particularly difficult to e ...

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Gas Chromatography-High-Resolution Mass Spectrometry-Based Method for the Simultaneous Determination of Organotin Compounds in Water

A gas chromatography-high-resolution mass spectrometry (GC-HRMS)-based method has been developed for the accurate and sensitive determination of nine organotin compounds in water: tetrabutyltin, tributyltin, dibutyltin, monobutyltin, triphenyltin, diphenyltin, m ...

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Transcription Start-Site Mapping

he synthesis of proteins is an essential process in cell growth and cell proliferation. The DNA sequence of a gene is first copied (transcribed) into an RNA sequence that is translated into a particular amino acid sequence. One strand of the gene, the RNA-like strand (or sense strand) has a nucleotide se ...

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Isolation of Campylobacter and Identification byPCR

Campylobacter is now recognized worldwide as a leading cause of bacterial gastroenteritis in humans (1). Campylobacter species are common commensals in the intestinal tracts of poultry and livestock, and food products of animal origin are frequently associated with reported cases ...

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Blocking Immunodominant Epitopes by Competitive Deselection

The development of combinatorial antibody (Ab) libraries displayed on the surface of phage has led to the production of a wide range of human monoclonal antibodies (MAbs) against a plethora of viral antigens (Ag) (1-5). However, sometimes the isolation of a given Ab can be particularly difficult ...

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Periplasmic Expression and Purification of Recombinant Fabs

The ability to refine the affinity, specificity, and immunogenicity of recombinant antibodies (Abs) offers distinct advantages for the preparation of in vivo diagnostic and therapeutic immunoreagents. The bacterial expression vector pComb3 allows production of recombina ...

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Bacterial Genomes for the Masses: Relevance to the Clinical Laboratory

Bacterial genome sequencing has revolutionized the research landscape and promises to deliver important changes to the clinical microbiology laboratory, through the identification of novel diagnostic targets and through the birth of a new discipline or “genomic epidemiolo ...

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Determination of rRNA Gene Restriction Patterns

The development of molecular biology has opened the way to new approaches to bacterial identification and typing. Nucleic acids carry the information encoding the bacterial diversity. They can be sequenced easily and, even when undetermined, their sequences can be quickly compared by m ...

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Application of Total DNA Restriction Pattern Analysis to Identification and Differentiation of Bacterial Strains

Total DNA restriction pattern analysis is one of the techniques commonly used to identify and group bacteria (1–11). This technique can be used to separate species belonging to the same genus (12,13) or to distinguish highly similar bacterial strains within the same species or subspecies (14– ...

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