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Analyzing Apoptosis in Cultured Epithelial Cells

This chapter will outline a number of assays in use in the authors′ laboratory for the quantification of apoptosis in cultured mammary epithelial cells. These assays are each directed at particular stages of apoptosis, which are outlined below. There are a number of commercial assays availab ...

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Isolation, Cultivation, and Differentiation of Normal Human Epidermal Keratinocytes in Serum-Free Medium

The outer layer of human skin is the epidermis. More than 90% of the epidermal cells are keratinocytes, which originate from stem cells, located in the basal cell layer (Stratum basale). Keratinocytes in the basal layer divide, differentiate, and migrate to the outer layer of the epidermis, the horny ...

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Epithelial Cell Integrins

Integrin receptors mediate the adhesion of cells to the extracellular matrix and to other cells. They play a significant role in a variety of biological functions

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Clinical Application of Autologous Cultured Epithelia for the Treatment of Burns and Disfigurement of Skin Surfaces

In patients who sustain large burns, it is difficult to cover the wounds with the patient’s own skin. Therefore, various materials such as allo-skin, porcine skin, and artificial skin have been used as biological dressings. However, only the patient’s own skin can survive permanently and succeed ...

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Transplantation of Cultured Epithelial Cells

Cultured human epithelium (autografts) have been used su+ccessfully since 1980 as a permanent cover for large full skin thickness burn wounds (1,1. They were also applied successfully to treat traumatic scars, giant nevi, and tattoo (3,1). The use of allogenic cultured epithelium (allogr ...

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An Outgrowth Culture System of Normal Human Epithelium: An In Vitro Model to Study the Coordination of Cellular Migration and Proliferation

In order to study the growth regulation of normal human epithelium, we have developed an outgrowth culture system (1). Cinematographic observation has revealed that the cells migrate and proliferate in a coordinated manner. For example, an outgrowth will expand concentrically as a cell s ...

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Human Lens Epithelial Cell Culture

Crystalline lens consists of epithelial cells, fiber cells, and a capsule. They originate from lens epithelial cells. Epithelial cells differentiate into fiber cells and produce collagen, which is the major compound of the capsule. The lens epithelial cells maintain normal physiology ...

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Applications of Epithelial Cell Culture in Studies of Drug Transport

The popularity of epithelial cell lines in studies of drug transport processes can probably be explained by the ease with which new information is derived from these rather simple in vitro models. Drug transport studies in epithelial cell monolayers grown on permeable supports are easy to pe ...

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Application of Epithelial Cell Culture in Drug Transport in the Respiratory Tract

Drugs can be inhaled and absorbed throughout the conducting airway from the trachea down to the bronchioles and ultimately in the distal lung across the alveolar epithelium. The respiratory tract offers a large surface area, extensive vasculature, and significantly lower enzymatic a ...

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X-Ray Microanalysis of Epithelial Cells in Culture

X-ray microanalysis is a technique of elemental analysis that is carried out in an electron microscope. The technique is based on the generation of characteristic X-rays in atoms of the specimen by the incident beam electrons. These X-rays are characteristic for the element from which they ori ...

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ENU Mutagenesis of Rat Mammary Epithelial Cells

Studies of the rat mammary carcinogenesis model have provided useful information on the mechanisms of human breast cancer. The rat model demonstrated that chemical carcinogens are causative agents of mammary cancer and that initiation of cancer requires the interaction of the carci ...

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Measurement of Membrane Capacitance in Epithelial Monolayers

Biological membranes serve as physical barriers and electrical insulators to separate different aqueous compartments, such as the intracellular and extracellular environments. In electrical terms, membranes that separate highly conductive aqueous media represent ca ...

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ENU-Induced Ovarian Cancer

Carcinogenesis, in both humans and experimental animals, is recognized to represent a multistep process involving a sequence of morphophenotypical changes, which may lead to malignant transformation. Various in vivo and in vitro models were developed to better mechanistically u ...

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Co-Cultivation of Liver Epithelial Cells with Hepatocytes

Primary hepatocytes from rat and other species maintained in pure monolayer culture undergo phenotypic changes within a few days (1-3). This is particularly pronounced with respect to the cytochrome P450-dependent biotransformation capacity, but may also involve other metabol ...

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Assessing Epithelial Cell Confluence by Spectroscopy

There is an increasing use of epithelial cells grown on tissue culture supports. Such tissue culture systems are used to study the cellular mechanisms involved in protein and lipid sorting between the two series membranes, which comprise the polarized epithelia cell, the molecular biolo ...

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Co-Culture and Crosstalk between Endothelial Cells and Vascular Smooth Muscle Cells Mediated by Intracellular Calcium

This chapter aims to describe the basic procedure needed to establish primary cultures of vascular smooth muscle cells (VSMCs) (1), and vascular endothelial cells (ECs) (2). Direct visualization of crosstalk between two types of cell was first accomplished by means of 2-dimensional Ca2+i ...

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Establishing Epithelial-Immune Cell Co-Cultures: Effects on Epithelial Ion Transport and Permeability

The epithelial lining of the gastrointestinal tract is constantly exposed to a vast array of antigenic and potentially disease-evoking material derived from the diet and gut microflora. Thus, this single cell thick layer of cells (mainly transporting enterocytes, but also mucin-sec ...

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Human Airway Epithelial Cell Culture

Development of methods to culture airway epithelial cells has been needed to carry out research into various lung diseases, such as cancer, cystic fibrosis, and bronchial asthma. However, the culture of airway epithelial cells remained difficult. We have improved the culture conditions ...

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Explant Cultures of Embryonic Epithelium: Analysis of Mesenchymal Signals

The development of most organs is characterized by epithelial morphogenesis involving budding, branching, and folding of the epithelium, which is accompanied by growth and differentiation of the epithelial cells. Central mechanisms regulating this development are interact ...

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Bacterial Interactions with Host Epithelium In Vitro

During recent years there has been a resurgence of interest in the role that bacteria, both pathogenic and commensal, play in the pathogenesis and pathophysiology of disease. For example, antibiotics can be a useful therapy to relieve the symptomatology experienced by a cohort of patients wi ...

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