丁香实验_LOGO
登录
提问
提问
我要登录
|免费注册
丁香实验推荐阅读
Chromatin Immunoprecipitation Assays: Application of ChIP-on-Chip for Defining Dynamic Transcriptional Mechanisms in Bone Cells

Normal cell growth and differentiation of bone cells requires the sequential expression of cell type specific genes to permit lineage specification and development of cellular phenotypes. Transcriptional activation and repression of distinct sets of genes support the anabol ...

丁香实验推荐阅读
Identification of Transcription Factor Target Genes by ChIP Display

Transcription factors play pivotal roles in the control of cell growth and differentiation in health and disease. In the post-genomic era, it has become possible to locate the regions occupied by transcription factors throughout the genome, leading to better understanding of their mech ...

丁香实验推荐阅读
Application of the Laser Capture Microdissection Technique for Molecular Definition of Skeletal Cell Differentiation In Vivo

Laser capture microdissection (LCM) method allows selection of individual or clustered cells from intact tissues. This technology enables one to pick cells from tissues that are difficult to study individually, sort the anatomical complexity of these tissues, and make the cells avail ...

丁香实验推荐阅读
In Situ Nuclear Organization of Regulatory Machinery

Regulatory machinery for gene expression, replication, and repair are architecturally organized in nuclear microenvironments. This compartmentalization provides threshold concentrations of macromolecules for the organization and assembly of regulatory compl ...

丁香实验推荐阅读
Quantitative Trait Loci Mapping

This chapter presents current methods for mapping quantitative trait loci (QTLs) in natural populations especially in humans. We discussed the experimental designs for QTL mapping, traditional methods adopted such as linkage mapping approaches and methods for linkage disequi ...

丁香实验推荐阅读
Bioluminescent Imaging in Bone

Monitoring gene expression in vitro and in vivo, is crucial when analyzing osteogenesis and developing effective bone gene therapy protocols. Until recently, molecular analytical tools were only able to detect protein expression either in vitro or in vivo. These systems include histo ...

丁香实验推荐阅读
Micro-Computed Tomography: A Method for the Non-Destructive Evaluation of the Three-Dimensional Structure of Biological Specimens

The large increase in interest in micro-computed tomography (micro-CT) over the last decade reflects the+ need for a method able to non-destructively visualize the internal three-dimensional structure of an object. Thereby, the real beauty of computed tomography lies in the fact that it is a ...

丁香实验推荐阅读
Fluid Flow Assays

Mechanical signals are major regulators of skeletal homeostasis as the addition of exogenous load is followed by enhanced bone formation and the removal of normal loads is followed by net bone loss. The mechanism by which bone cells perceive and respond to changes in their biophysical enviro ...

丁香实验推荐阅读
Noninvasive Study of Human Cartilage Structure by MRI

Magnetic resonance (MR) imaging is a 3D imaging technique that has recently begun to permit direct delineation of cartilage structure. This chapter summarizes current methodology for the morphological (e.g., volume, thickness) and compositional imaging of cartilage using quant ...

丁香实验推荐阅读
High-Resolution MRI Techniques for Studies in Small-Animal Models

High-field magnetic resonance (MR), which allows high-resolution imaging, is a powerful research tool to examine and visualize hyaline cartilage of small joints noninvasively. Recent studies have shown that qualitative assessment of degenerative joint disease, derived from MR ...

丁香实验推荐阅读
High-Resolution Imaging of Osteoarthritis Using Microcomputed Tomography

Three-dimensional imaging of osteoarthritis is so far limited to late stages of the disease. In this chapter we introduce microcomputed tomography (�CT) as a new imaging tool that offers exciting features for diagnosis of earlier disease stages and for disease monitoring. �CT provides sp ...

丁香实验推荐阅读
High-Resolution Ultrasonography for Analysis of Age- and Disease-Related Cartilage Changes

Because of their limited spatial resolution, current clinical noninvasive imaging modalities (radiography, computed tomography, conventional echography, and magnetic resonance imaging) are able to detect only the late stages of the cartilage degradation. To detect early le ...

丁香实验推荐阅读
Evaluation of Cartilage Composition and Degradation by High-Resolution Magic-Angle Spinning Nuclear Magnetic Resonance

Rheumatic diseases are accompanied by a progressive destruction of the cartilage layers of the joints. Although the number of patients suffering from rheumatic diseases is steadily increasing, degradation mechanisms of cartilage are not yet understood, and methods for early diag ...

丁香实验推荐阅读
Pulsed-Field Gradient-Nuclear Magnetic Resonance (PFG NMR) to Measure the Diffusion of Ions and Polymers in Cartilage: Applications in Joint Diseases

Since cartilage contains neither blood nor lymph vessels, diffusion is the most important transport process for the supply of cartilage with nutrients and for the removal of metabolic waste products. Therefore, diffusion measurements are of high interest in cartilage research. Diff ...

丁香实验推荐阅读
Dynamics of Collagen in Articular Cartilage Studied by Solid-State NMR Methods

Methods for studying the fast molecular dynamics of the rigid macromolecules in cartilage are described. The strong dipolar couplings and chemical shift anisotropies of these molecules necessitate application of solid-state nuclear magnetic resonance (NMR) techniques such ...

丁香实验推荐阅读
Computerized Protocol for Anatomical and Functional Studies of Joints

This chapter describes a new methodology for the acquisition and computer elaboration of joint anatomy and motion data and the study of their correlation. The method uses a commercial industrial electrogoniometer, custom tools, and software designed and developed by the authors for in ...

丁香实验推荐阅读
Generation and Use of Transgenic Mice As Models of Osteoarthritis

The term osteoarthritis (OA) represents a group of diseases characterized by gradual degradation of articular cartilage and a number of associated degenerative processes within the joint. Consequently, no single animal model is likely to fulfil all the criteria of OA. The present chapt ...

丁香实验推荐阅读
Development and Clinical Application in Arthritis of a New Immunoassay for Serum Type IIA Procollagen NH2 Propeptide

Type II collagen, the most abundant protein of cartilage matrix, is synthesized as a procollagen molecule including the N-(PIINP) and C-(PIICP) propeptides at each end. Type II procollagen is produced in two forms as the result of alternative RNA splicing. One form (IIA) includes and the other form ( ...

丁香实验推荐阅读
Histological and Immunohistological Studies on Cartilage

The material properties of dense avascular connective tissues such as cartilage present unique challenges to the development of any prospective histological procedures. This chapter documents some of the protocols we have developed in our laboratories for the histological and i ...

丁香实验推荐阅读
Histochemical Visualization of the Cartilage Hyaladherins Using a Biotinylated Hyaluronan Oligosaccharide Bioaffinity Probe

Hyaluronan (HA) binding proteins (HABPs) were localized in cartilaginous ovine tissues (articular cartilage, intervertebral disc) using a biotinylated HA (bHA) oligosaccharide bioaffinity probe. The bHA oligosaccharide probe was prepared by partial digestion of HA with ovi ...

提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
关注公众号
反馈
TOP
打开小程序