提问
提问
我要登录
|免费注册
丁香实验推荐阅读
Quantum Dots for Labeling Live Cells

Quantum dots (QDs) are semiconductor nanocrystals with unique photo-physical properties. QDs are brightly fluorescent and photostable, and therefore represent a novel class of fluorescence probes for bio-imaging and cell monitoring. There are different techniques for label ...

丁香实验推荐阅读
The Use of Quantum Dots for Immunochemistry Applications

Immunocytochemistry and histochemistry are two most valuable immunochemistry techniques routinely used in biological laboratories. These techniques rely on the use of antibodies to label epitopes of interest in cells. At present, there is a wide range of commercially available o ...

丁香实验推荐阅读
Oxidative Modifications of Mitochondria Complex II

Increased superoxide (O2 •−) and nitric oxide (NO) production is a key mechanism of mitochondrial dysfunction in myocardial ischemia/reperfusion injury. In the complex II, oxidative impairment, decreased protein S-glutathionylation, and increased protein tyrosine nitrat ...

丁香实验推荐阅读
Proteomic Analysis of Brain Mitochondrial Proteome and Mitochondrial Complexes

We describe various complementary techniques to achieve multidimensional mitochondrial proteome fractionation and analysis. Previously described methods for 2D-DIGE/mass spectrometry and 1D-SDS-PAGE/Western techniques and protein complex analysis by BN-PAGE/ ...

丁香实验推荐阅读
Characterization of Zebrafish Cardiac Proteome Using Online pH Gradient SCXRP HPLCMS/MS Platform

Two-dimensional HPLC coupled with tandem MS (MS/MS) has become a mainstream technique in the shotgun proteomics for large-scale identification of proteins from biological samples. This powerful technology provides speed, sensitivity, and dynamic range which are essential to pr ...

丁香实验推荐阅读
Suction Blister Model of Wound Healing

The suction blister model was originally developed for the separation of viable epidermis from dermis by Kiistala (1). Since its development, its use has been expanded and applied to several other applications. One new application assesses the collagen synthesis rate in the human skin in vivo ...

丁香实验推荐阅读
Specimen Collection and Analysis: Burn Wounds

The incidence of fire deaths in the United States has decreased from 2.5/100,000 population in 1983 to 1.7/100,000 in 1993 (1). In the last century, tremendous strides have occurred in the acute resuscitation of burns, permitting burn clinicians and researchers to concentrate on improving fun ...

丁香实验推荐阅读
Wound Repair in Aging: A Review

The purpose of this chapter is to review the changes in wound healing that occur in the aged. Unlike pathological conditions such as infection or diabetes as a cause for impaired wound repair, aging may simply reduce the speed at which an individual heals. Thus, a goal of this review is to assess whether aging ...

丁香实验推荐阅读
Wound Healing Studies in Transgenic and Knockout Mice: A Review

Wound healing is a highly ordered and well coordinated process that involves inflammation, cell proliferation, matrix deposition, and tissue remodeling (1). During the past few years, a series of candidate key players in the wound-healing scenario have been identified. These include not ...

丁香实验推荐阅读
Tissue Repair in Models of Diabetes Mellitus: A Review

Diabetes mellitus is a major cause of impaired tissue repair. Patients with this disease not only have a propensity to develop wounds, but when they do, they tend to have difficulty healing those wounds. Simple wounds often become chronic and infectious wound complications are not uncommon. Un ...

丁香实验推荐阅读
Growth of Human Blood Vessels in Severe Combined Immunodeficient Mice: A New In Vivo Model System of Angiogenesis

The development of strategies for the treatment of angiogenesis-dependent diseases has been greatly aided by the development of in vivo models of angiogenesis (1). These bioassays provide investigators with tools to visualize vessel architecture and function and to analyze and man ...

丁香实验推荐阅读
Methods for Investigating Fetal Tissue Repair

This chapter is devoted to a fascinating topic in molecular biology—scarless fetal wound repair. Our understanding of this phenomenon may one day allow us to manipulate the adult wound-healing process to recapitulate the scarless phenotype. To achieve this goal, we must first understand ...

丁香实验推荐阅读
Murine Model of Peritoneal Adhesion Formation

The lining of the organs within the peritoneal cavity consists of a single layer of mesothelial cells with a minimum of underlying connective tissue. This same cellular structure covers the luminal surface of the abdominal wall musculature. Together these mesothelial layers serve as a smo ...

丁香实验推荐阅读
Murine Models of Intestinal Anastomoses

Wound healing is a physiological process that is essential to the reestablishment of homeostasis (1-4). It is generally accepted that wound repair is an immune-mediated event (5) that involves a number of cell types such as macrophages, neutrophils, fibroblasts, endothelial cells, and ke ...

丁香实验推荐阅读
Subcutaneous Sponge Models

All wounds progress through a well-described series of events that ultimately result in healing. Cellular, biochemical, and molecular changes occur, each characterizing unique phases of the healing process. Scientists have long desired to study both the global process of healing and i ...

丁香实验推荐阅读
Corneal Injury: A Relatively Pure Model of Stromal-Epithelial Interactions in Wound Healing

Wound healing is a complex cascade that varies depending on the organ being studied. However, many components of the response are similar in different organs. Stromal-epithelial interactions are critical to wound healing in tissues in which epithelium and stroma are conjoined. The corn ...

丁香实验推荐阅读
Animal Models of Ischemic Wound Healing: Toward an Approximation of Human Chronic Cutaneous Ulcers in Rabbit and Rat

Chronic wounds are defined as wounds that heal with a significant delay, usually over a period of more than 2 mo. The morbidity associated with delayed wound healing imposes an enormous social and financial burden on the health care system. Clinical observations suggest that persistent tiss ...

丁香实验推荐阅读
Incisional Wound Healing: Model and Analysis of Wound Breaking Strength

The nature and mechanism of incisional wound healing has been and continues to be of interest to clinicians and wound biologists. More than 46 million operations are performed in the United States alone each year (1). To shorten the time required for incisional wound healing is not only relevant to r ...

丁香实验推荐阅读
Methods in Reepithelialization: A Porcine Model of Partial-Thickness Wounds

The healing of skin wounds progresses through sequential and overlapping phases of infl ammation, repair, and remodeling ( Fig. 1A ). Each phase of healing is directed by the complex coordination and interaction of several cell types contained within the wound, including inflammatory cel ...

丁香实验推荐阅读
In Vitro Matrigel Angiogenesis Model

Angiogenesis, the formation of new blood vessels, is a key process in wound healing. During the angiogenic phase of injured tissue repair, epithelial cells sprout and branch from preexisting vessels, evolving into a network of new capillaries. This formation of vasculature, necessary for ...

提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
关注公众号
反馈
TOP
打开小程序