丁香实验_LOGO
登录
提问
我要登录
|免费注册
丁香实验推荐阅读
In Vitro Systems for Studying Epithelial Transport of Macromolecules

Biological barriers, typically, represented by epithelial tissues are the main hindrance against uncontrolled uptake of a variety of substances. However, the delivery across a biological barrier is a crucial factor in the development of drugs. As the permeability of macromolecular ...

丁香实验推荐阅读
Enhanced Delivery of Macromolecules into Cells by Electroendocytosis

Transfer of exogenous material into the cytosol of cells is one of the main challenges in drug delivery. We present a novel physical approach for efficient incorporation of macromolecules into living cells, based on exposing them to a train of unipolar electric field pulses, possessing much l ...

丁香实验推荐阅读
Guidelines for the Selection of Effective Short-Interfering RNA Sequences for Functional Genomics

To avoid long-double-stranded-RNA-dependent interferon response, short-interfering RNAs (siRNAs) are widely used for RNA interference (RNAi) in mammalian cells. siRNA-based RNAi, however, may not be readily available for the large-scale gene silencing essential for system ...

丁香实验推荐阅读
Nucleic Acid-Based Aptamers as Promising Therapeutics in Neoplastic Diseases

Isolated through combinatorial libraries by an iterative in vitro selection process, small single-stranded nucleic acid compounds, named aptamers, have been developed as high-affinity ligands for a variety of targets, ranging from small chemical compounds to large proteins. In t ...

丁香实验推荐阅读
Human Esophageal Culture

This chapter describes a method for establishing short-term expiant cultures of esophageal mucosa. Adverse effects produced by exposure to radiation or test compounds can be detected as an inhibition of cell outgrowth.

丁香实验推荐阅读
Alternative Method of Assessing Toxicity

The safety assessment of new chemicals and pharmaceuticals and the incorporation of these data into a human risk assessment package requires a large number of expensive, regulated tests in animal species including, in some cases, nonhuman primates (1–3).

丁香实验推荐阅读
The Application of In Vitro Models of Hypothalamic Function in Toxicity Testing

The reader is referred to the previous chapter in which the background and rationale for monitoring hypothalamic function in vitro is discussed.

丁香实验推荐阅读
The Application of In Vitro Models of Anterior Pituitary Function in Toxicity Testing

Together, the hypothalamus and the pituitary gland form the functional unit called the neuroendocrine system. For the purposes of introducing this system, therefore, the two will be considered together, although the methods for assessing their function will be considered separate ...

丁香实验推荐阅读
Human Thyroid Culture

The technique described in this chapter enables the culturing of thyroid cells without loss of differentiation and medium change. It is potentially useful for the long-term study of drug effects on the thyroid gland. Human thyroid cells obtained during surgery can be maintained in culture f ...

丁香实验推荐阅读
Preparation and Use of Cultured Astrocytes for Assay of Gliotoxicity

Cultured astrocytes provide a valuable and important system for predictive testing and mechanistic analysis of neurotoxic compounds. The culture procedures allow relatively rapid assessment of different chemicals or their metabolites over a range of concentrations, using c ...

丁香实验推荐阅读
LLC-RK1 Cell Screening Test for Nephrotoxicity

In this test, kidney-derived cells are cultured in the presence of test compounds whose cytotoxicity is then determined by the Neutral Red method, and serves as an indicator of potential nephrotoxicity (1). Healthy LLC-RK1 cells (an established cell line, ATCC CCL) maintained in culture cont ...

丁香实验推荐阅读
Thyroid Follicular Cells in Monolayer Culture: In Vitro Models for Thyroid Toxicity Testing

The development of in vitro models that preserve the functional characteristics of the normal thyroid gland has been a challenging objective of recent experimental thyroidology. A major aim of such endeavors has been to facilitate the development of simple, reliable, reproducible te ...

丁香实验推荐阅读
Dust Toxicity in Rat Alveolar Macrophage Cultures

Macrophage cells in culture may be exposed to particulate matter, and resultant effects on cell viability are determined by vital dye exclusion and enzyme leakage assays.

丁香实验推荐阅读
Cytotoxicity, DNA Fragmentation, and DNA Repair Synthesis in Primary Human Hepatocytes

The well-documented existence of interspecies differences in the responses elicited by exposure to chemical agents (1–3) represents the main difficulty in the extrapolation to humans of the results of toxicity studies carried out in laboratory animals or in in vitro systems employing ...

丁香实验推荐阅读
Hepatoma Cell Cultures as In Vitro Models for Hepatotoxicity

This test is designed to detect irreversible toxic effects on both cell growth and survival, by the evaluation of colony-forming (CF) efficiency, in hepatoma cell lines derived from humans, rat, and mouse. The liver is a major target organ for the cytotoxicity of many xenobiotics. It has been sugges ...

丁香实验推荐阅读
Model Cavity Method: Invittox

The model cavity method enables the in vitro cytotoxicity testing of dental restorative materials that may then be related to dental toxicity likely to occur in vivo.

丁香实验推荐阅读
The FRAME Cytotoxicity Test (Kenacid Blue)

The cytotoxic effect of chemicals on cells in culture is measured by the change in total cell protein (Kenacid Blue R dye binding method). Healthy 3T3-L1 cells (an established cell-line, ATCC CCL92.1), when maintained in culture continuously divide and multiply over time. The basis of this test is t ...

丁香实验推荐阅读
Measurement of Cell Membrane Toxicity by Means of 2-Deoxy-D-Glucose

The membrane theory of toxicity (1) emanates from the fact that the plasma membrane is the first barrier met by a toxic agent on reaching the cell, and was first advanced to explain the toxic effects of heavy metals. The vital regulatory mechanisms inherent in the plasma membrane and its chemical compo ...

丁香实验推荐阅读
Allium Test

The Allium test provides a rapid screening procedure for chemicals, pollutants, contaminants, and so on that may represent environmental hazards. Root growth inhibition and adverse effects on chromosomes provide an indication of likely toxicity.

丁香实验推荐阅读
MTT Assays

The MTT calorimetric assay determines the ability of viable cells to convert a soluble tetrazolium salt (MTT) into an insoluble formazan precipitate. Tetrazolium salts accept electrons from oxidized substrates or appropriate enzymes, such as NADH and NADPH. In particular, MTT is redu ...

提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
扫码领资料
反馈
TOP
打开小程序