提问
提问
我要登录
|免费注册
丁香实验推荐阅读
In Situ Hybridization with Isotopic Riboprobes for Detection of Striatal Neuropeptide mRNA Expression After Dopamine Stimulant Administration

Stimulant drugs such as cocaine, amphetamine, and methylphenidate induce their primary pharmacological actions through elevation of dopamine levels in the brain. The most dense innervation of dopamine nerve terminals in the central nervous system (CNS) is found within the striatum ...

丁香实验推荐阅读
Quantification of Protein in Brain Tissue by Western Immunoblot Analysis

Western blots are designed to determine protein levels and their patterns of modification in homogenized tissue samples. Western blots are quantifiable, but unlike immunohistochemistry, the cellular integrity is lost. Both Western blots and immunohistochemistry depend on the ...

丁香实验推荐阅读
Combining In Situ Hybridization with Retrograde Tracing and Immunohistochemistry for Phenotypic Characterization of Individual Neurons

In situ hybridization, using radioactive and nonradioactive probes, is at present, a widespread technique that has been developed and improved over the past 15 yr, and has become an extremely powerful tool for the study of cellular interactions through gene expression and gene regulation. ...

丁香实验推荐阅读
Immunohistochemical and Immunocytochemical Detection of Phosphoproteins in Striatal Neurons

Phosphorylation of protein kinases and transcription factors represents a major biological mechanism to activate those imperative intracellular effectors. Examination of such phosphorylation in response to extracellular stimulation can assess the functional part ...

丁香实验推荐阅读
Analysis of Protein Expression in Brain Tissue by ELISA

The enzyme-linked immunosorbent assay (ELISA) technique offers a sensitive, simple, and versatile method for quantifying as little as 100 pg of target protein in mixed cell or tissue lysates. A further advantage to the protocol is the ability to process rapidly and reproducibly large numbe ...

丁香实验推荐阅读
Analysis of DNA-Binding Activity in Neuronal Tissue with the Electrophoretic Mobility-Shift Assay

DNA is bound by many proteins in a sequence specific manner. Electrophoretic mobility-shift assays (EMSAs), also known as gel shift assays, are designed to determine the amount and identity of proteins from a particular sample that bind to a specific DNA sequence, usually an enhancer element. T ...

丁香实验推荐阅读
Dopamine Receptor Binding and Quantitative Autoradiographic Study

The central nervous system (CNS) dopamine system plays an important role in mediating the reinforcing effects of drugs of abuse (1). In addition, dopamine receptors have been the principal target of drugs employed in the treatment of neuropsychiatric disorders such as schizophrenia and P ...

丁香实验推荐阅读
Viral-Mediated Gene Transfer to Study the Behavioral Correlates of CREB Function in the Nucleus Accumbens of Rats

There is an enormous initiative to establish causal relationships between brain biology (including patterns of gene expression) and behavior. Unfortunately, genetic intervention is not accomplished easily in the brain. One strategy is to engineer and deliver to the brain speciali ...

丁香实验推荐阅读
Artificial Membrane Models for the Study of Macromolecular Delivery

Artificial biomembrane mimetic model systems are used to characterize peptide–membrane interactions using a wide range of methods. Herein, we present the use of selected membrane model systems to investigate peptide–membrane interactions. We describe methods for the prepara ...

丁香实验推荐阅读
Selection and Characterization of Antibodies from Phage Display Libraries Against Internalizing Membrane Antigens

Macromolecular delivery systems require high target cell specificity and efficient intracellular uptake. Monoclonal antibodies (mAbs) have been shown to successfully meet these needs and should, due to their biological nature and thus minimal toxicity and limited immunogen ...

丁香实验推荐阅读
Characterizing Peptide-Mediated DNA Internalization in Human Cancer Cells

Cell penetrating peptides (CPPs) are currently used to deliver various macromolecular cargos to intracellular sites of action both in vitro and in vivo on an experimental basis. During the last few years, even more evidence has accumulated indicating that the main route of entry for most CPPs is ...

丁香实验推荐阅读
Peptide-Based Delivery of Steric-Block PNA Oligonucleotides

Several strategies based on synthetic oligonucleotides (ON) have been proposed to control gene expression. As for most biomolecules, however, delivery has remained a major roadblock for in vivo applications. Conjugation of steric-block neutral DNA mimics such as peptide nucleic ac ...

丁香实验推荐阅读
Systemic Delivery and Pre-clinical Evaluation of Nanoparticles Containing Antisense Oligonucleotides and siRNAs

By virtue of their potential to selectively silence oncogenic molecules in cancer cells, antisense oligonucleotides (ASO) and small interfering RNAs (siRNAs) are powerful tools for development of tailored anti-cancer drugs. The clinical benefit of ASO/siRNA therapeutic is, how ...

丁香实验推荐阅读
Poly(-amino esters): Procedures for Synthesis and Gene Delivery

Non-viral gene delivery systems are promising as they avoid many problems of viral gene therapy by having increased design flexibility, high safety, large DNA cargo capacity, and ease of manufacture. Here, we describe the use of polymeric vectors, in particular biodegradable poly(β-ami ...

丁香实验推荐阅读
siRNA and DNA Transfer to Cultured Cells

Transfection is a powerful non-viral technology used to deliver foreign nucleic acids into eukaryotic cells, and is the method of choice for a variety of applications including studying the functional role of particular genes and the proteins they code for. By over-expressing genes to prod ...

丁香实验推荐阅读
Synthetic vs. Natural/Biodegradable Polymers for Delivery of shRNA-Based Cancer Therapies

DNA vector-based short hairpin RNA (shRNA) as a means of effecting RNA interference (RNAi) is a promising mechanism for the precise disruption of gene expression to achieve a therapeutic effect. The clinical usage of shRNA therapeutics in cancer is limited by obstacles related to effective d ...

丁香实验推荐阅读
Developments in Macromolecular Drug Delivery

Macromolecular drugs hold great promise as novel therapeutics of several major disorders, such as cancer and cardiovascular disease. However, their use is limited by lack of efficient, safe, and specific delivery strategies. Successful development of such strategies requires int ...

丁香实验推荐阅读
Positron Emission Tomography (PET) and Macromolecular Delivery In Vivo

Positron emission tomography (PET) examinations with F-18-fluorodeoxyglucose (FDG) provide detailed information about the glucose-like metabolism in tissue. It is generally accepted that FDG reflects the viability of tumour cells. The kinetics of FDG is modulated by several ge ...

丁香实验推荐阅读
Macromolecular Delivery Across the BloodBrain Barrier

The delivery of macromolecules into the central nervous system (CNS) via the blood stream is seriously limited by the blood–brain barrier (BBB). Noninvasive, transient, and local image-guided blood–brain barrier disruption (BBBD) can be accomplished using focused ultrasound exp ...

丁香实验推荐阅读
Preparation of Macromolecule-Containing Dry Powders for Pulmonary Delivery

Drug delivery by inhalation is routine for the treatment of local pulmonary conditions like asthma, cystic fibrosis, and chronic obstructive pulmonary disease. Only recently, though, has the inhalation route been considered for administering drugs for systemic diseases. The pulm ...

提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
关注公众号
反馈
TOP
打开小程序