Regulation of Chromatin Structure by PARP-1

互联网2014-02-13

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PARP-1 is the most abundantly expressed member of a family of proteins that catalyze the transfer of ADP-ribose units from NAD⁺ to target proteins. Numerous studies on PARP-1 have revealed its critical roles in regulating various molecular and cellular processes including DNA damage detection and repair, chromatin modification.

In this chapter, we describe various in vitro methods to investigate the function of PARP-1 in the regulation of chromatin structure using chromatin templates.

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