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Strategies for Large-Scale Production of Recombinant Proteins in Filamentous Fungi

互联网

562
A pilot-plant process for the production of a recombinant sweet-tasting protein in the filamentous fungus Aspergillus awamori is described. Each step of the scale-up and downstream processes (concentration, diafiltration and ion-exchange chromatography) for the production and purification of the secreted recombinant protein is detailed.
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