让人流口水的好书

Protein–Protein Interactions ：Methods and Applications March 2004

http://biomed.humanapress.com/BookTOC.pasp?isbn=1-59259-762-9&returntoisbn=
Part I. Overview

Structural Basis of Protein–Protein Interactions
Robert C. Liddington

Quantitative Analysis of Protein–Protein Interactions
Keith D. Wilkinson

Part II. In Vitro Techniques

Characterization of Protein–Protein Interactions by Isothermal Titration Calorimetry
Adrian Velazquez-Campoy, Stephanie A. Leavitt, and Ernesto Freire

Circular Dichroism Analysis for Protein–Protein Interactions
Norma J. Greenfield

Protein–Protein Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy
Guanghua Gao, Jason G. Williams, and Sharon L. Campbell

Measuring Rhodopsin–G-Protein Interactions by Surface Plasmon Resonance
John Northup

Using Light Scattering to Determine the Stoichiometry of Protein Complexes
Jeremy Mogridge

Sedimentation Equilibrium Studies
Ian A. Taylor, John F. Eccleston, and Katrin Rittinger

Analysis of Protein–Protein Interactions by Simulation of Small-Zone Gel Filtration Chromatography
Rosemarie Wilton, Elizabeth A. Myatt, and Fred J. Stevens

Fluorescence Gel Retardation Assay to Detect Protein–Protein Interactions
Sang-Hyun Park and Ronald T. Raines

Fluorescence Polarization Assay to Quantify Protein–Protein Interactions
Sang-Hyun Park and Ronald T. Raines

Studying Protein–Protein Interactions via Blot Overlay or Far Western Blot
Randy A. Hall

Glutathione-S-Transferase–Fusion-Based Assays for Studying Protein–Protein Interactions
Haris G. Vikis and Kun-Liang Guan

Affinity Capillary Electrophoresis Analyses of Protein–Protein Interactions in Target-Directed Drug Discovery
William E. Pierceall, Lixin Zhang, and Dallas E. Hughes

Mapping Protein–Ligand Interactions by Hydroxyl-Radical Protein Footprinting
Nick Loizos

Use of Phage Display and Polyvalency to Design Inhibitors of Protein–Protein Interactions
Michael Mourez and R. John Collier

Part III. Detecting Protein–Protein Interactions in Heterologous Systems

A Bacterial Two-Hybrid System Based on Transcriptional Activation
Simon L. Dove and Ann Hochschild

Using the Yeast Two-Hybrid System to Identify Interacting Proteins
John Miller and Igor Stagljar

Analysis of Protein–Protein Interactions Utilizing Dual Bait Yeast Two-Hybrid System
Ilya G. Serebriiskii and Elena Kotova

The Split-Ubiquitin Membrane-Based Yeast Two-Hybrid System
Safia Thaminy, John Miller, and Igor Stagljar

Reverse Two-Hybrid Techniques in the Yeast Saccharomyces cerevisiae
Matthew A. Bennett, Jack F. Shern, and Richard A. Kahn

Mammalian Two-Hybrid Assay for Detecting Protein–Protein Interactions In Vivo
Jae Woon Lee and Soo-Kyung Lee

Co-Immunoprecipitation from Transfected Cells
Shane C. Masters

Part IV: Probing Protein–Protein Interactions in Living Cells

Microscopic Analysis of Fluorescence Resonance Energy Transfer (FRET)
Brian Herman, R. V. Krishnan, and Victoria E. Centonze

Monitoring Molecular Interactions in Living Cells Using Flow Cytometric Analysis of Fluorescence Resonance Energy Transfer
Francis Ka-Ming Chan

Fluorescence Correlation Spectroscopy: A New Tool for Quantification of Molecular Interactions
Keith M. Berland

Confocal Microscopy for Intracellular Co-localization of Proteins
Toshiyuki Miyundefined*a~Kbr_~H~M~2~1~Kbr_~H~M~2~1Mapping_Biochemical_Networks_with_Protein~FFragment_Complementation_Assays~Kbr_~H~M~2~1Ingrid_Remy_and_Stephen_W._Michnick~Kbr_~H~M~2~1~Kbr_~H~M~2~1In_Vivo_Protein_Cross~FLinking~Kbr_~H~M~2~1Fabrice_Agou~E_Fei_Ye~E_and_Michel_Véron

Part V. Proteomics-Based Approaches

Computational Prediction of Protein–Protein Interactions
John C. Obenauer and Michael B. Yaffe

Affinity Methods for Phosphorylation-Dependent Interactions
Greg Moorhead and Carol MacKintosh

Two-Dimensional Gel Electrophoresis for Analysis of Protein Complexes
Karin Barnouin

Sample Preparation of Gel Electrophoretically Separated Protein Binding Partners for Analysis by Mass Spectrometry
Rainer Cramer, Malcolm Saxton, and Karin Barnouin

Quantitative Protein Analysis by Solid Phase Isotope Tagging and Mass Spectrometry
Huilin Zhou, Rosemary Boyle, and Ruedi Aebersold

Internet Resources for Studying Protein–Protein Interactions
Shane C. Masters

Index
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