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Protein–Protein Interactions :Methods and Applications March 2004
http://biomed.humanapress.com/BookTOC.pasp?isbn=1-59259-762-9&returntoisbn=
Part I. Overview
Structural Basis of Protein–Protein Interactions
Robert C. Liddington
Quantitative Analysis of Protein–Protein Interactions
Keith D. Wilkinson
Part II. In Vitro Techniques
Characterization of Protein–Protein Interactions by Isothermal Titration Calorimetry
Adrian Velazquez-Campoy, Stephanie A. Leavitt, and Ernesto Freire
Circular Dichroism Analysis for Protein–Protein Interactions
Norma J. Greenfield
Protein–Protein Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy
Guanghua Gao, Jason G. Williams, and Sharon L. Campbell
Measuring Rhodopsin–G-Protein Interactions by Surface Plasmon Resonance
John Northup
Using Light Scattering to Determine the Stoichiometry of Protein Complexes
Jeremy Mogridge
Sedimentation Equilibrium Studies
Ian A. Taylor, John F. Eccleston, and Katrin Rittinger
Analysis of Protein–Protein Interactions by Simulation of Small-Zone Gel Filtration Chromatography
Rosemarie Wilton, Elizabeth A. Myatt, and Fred J. Stevens
Fluorescence Gel Retardation Assay to Detect Protein–Protein Interactions
Sang-Hyun Park and Ronald T. Raines
Fluorescence Polarization Assay to Quantify Protein–Protein Interactions
Sang-Hyun Park and Ronald T. Raines
Studying Protein–Protein Interactions via Blot Overlay or Far Western Blot
Randy A. Hall
Glutathione-S-Transferase–Fusion-Based Assays for Studying Protein–Protein Interactions
Haris G. Vikis and Kun-Liang Guan
Affinity Capillary Electrophoresis Analyses of Protein–Protein Interactions in Target-Directed Drug Discovery
William E. Pierceall, Lixin Zhang, and Dallas E. Hughes
Mapping Protein–Ligand Interactions by Hydroxyl-Radical Protein Footprinting
Nick Loizos
Use of Phage Display and Polyvalency to Design Inhibitors of Protein–Protein Interactions
Michael Mourez and R. John Collier
Part III. Detecting Protein–Protein Interactions in Heterologous Systems
A Bacterial Two-Hybrid System Based on Transcriptional Activation
Simon L. Dove and Ann Hochschild
Using the Yeast Two-Hybrid System to Identify Interacting Proteins
John Miller and Igor Stagljar
Analysis of Protein–Protein Interactions Utilizing Dual Bait Yeast Two-Hybrid System
Ilya G. Serebriiskii and Elena Kotova
The Split-Ubiquitin Membrane-Based Yeast Two-Hybrid System
Safia Thaminy, John Miller, and Igor Stagljar
Reverse Two-Hybrid Techniques in the Yeast Saccharomyces cerevisiae
Matthew A. Bennett, Jack F. Shern, and Richard A. Kahn
Mammalian Two-Hybrid Assay for Detecting Protein–Protein Interactions In Vivo
Jae Woon Lee and Soo-Kyung Lee
Co-Immunoprecipitation from Transfected Cells
Shane C. Masters
Part IV: Probing Protein–Protein Interactions in Living Cells
Microscopic Analysis of Fluorescence Resonance Energy Transfer (FRET)
Brian Herman, R. V. Krishnan, and Victoria E. Centonze
Monitoring Molecular Interactions in Living Cells Using Flow Cytometric Analysis of Fluorescence Resonance Energy Transfer
Francis Ka-Ming Chan
Fluorescence Correlation Spectroscopy: A New Tool for Quantification of Molecular Interactions
Keith M. Berland
Confocal Microscopy for Intracellular Co-localization of Proteins
Toshiyuki Miyundefined*a~Kbr_~H~M~2~1~Kbr_~H~M~2~1Mapping_Biochemical_Networks_with_Protein~FFragment_Complementation_Assays~Kbr_~H~M~2~1Ingrid_Remy_and_Stephen_W._Michnick~Kbr_~H~M~2~1~Kbr_~H~M~2~1In_Vivo_Protein_Cross~FLinking~Kbr_~H~M~2~1Fabrice_Agou~E_Fei_Ye~E_and_Michel_Véron
Part V. Proteomics-Based Approaches
Computational Prediction of Protein–Protein Interactions
John C. Obenauer and Michael B. Yaffe
Affinity Methods for Phosphorylation-Dependent Interactions
Greg Moorhead and Carol MacKintosh
Two-Dimensional Gel Electrophoresis for Analysis of Protein Complexes
Karin Barnouin
Sample Preparation of Gel Electrophoretically Separated Protein Binding Partners for Analysis by Mass Spectrometry
Rainer Cramer, Malcolm Saxton, and Karin Barnouin
Quantitative Protein Analysis by Solid Phase Isotope Tagging and Mass Spectrometry
Huilin Zhou, Rosemary Boyle, and Ruedi Aebersold
Internet Resources for Studying Protein–Protein Interactions
Shane C. Masters
Index
真希望有看到的一天
http://biomed.humanapress.com/BookTOC.pasp?isbn=1-59259-762-9&returntoisbn=
Part I. Overview
Structural Basis of Protein–Protein Interactions
Robert C. Liddington
Quantitative Analysis of Protein–Protein Interactions
Keith D. Wilkinson
Part II. In Vitro Techniques
Characterization of Protein–Protein Interactions by Isothermal Titration Calorimetry
Adrian Velazquez-Campoy, Stephanie A. Leavitt, and Ernesto Freire
Circular Dichroism Analysis for Protein–Protein Interactions
Norma J. Greenfield
Protein–Protein Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy
Guanghua Gao, Jason G. Williams, and Sharon L. Campbell
Measuring Rhodopsin–G-Protein Interactions by Surface Plasmon Resonance
John Northup
Using Light Scattering to Determine the Stoichiometry of Protein Complexes
Jeremy Mogridge
Sedimentation Equilibrium Studies
Ian A. Taylor, John F. Eccleston, and Katrin Rittinger
Analysis of Protein–Protein Interactions by Simulation of Small-Zone Gel Filtration Chromatography
Rosemarie Wilton, Elizabeth A. Myatt, and Fred J. Stevens
Fluorescence Gel Retardation Assay to Detect Protein–Protein Interactions
Sang-Hyun Park and Ronald T. Raines
Fluorescence Polarization Assay to Quantify Protein–Protein Interactions
Sang-Hyun Park and Ronald T. Raines
Studying Protein–Protein Interactions via Blot Overlay or Far Western Blot
Randy A. Hall
Glutathione-S-Transferase–Fusion-Based Assays for Studying Protein–Protein Interactions
Haris G. Vikis and Kun-Liang Guan
Affinity Capillary Electrophoresis Analyses of Protein–Protein Interactions in Target-Directed Drug Discovery
William E. Pierceall, Lixin Zhang, and Dallas E. Hughes
Mapping Protein–Ligand Interactions by Hydroxyl-Radical Protein Footprinting
Nick Loizos
Use of Phage Display and Polyvalency to Design Inhibitors of Protein–Protein Interactions
Michael Mourez and R. John Collier
Part III. Detecting Protein–Protein Interactions in Heterologous Systems
A Bacterial Two-Hybrid System Based on Transcriptional Activation
Simon L. Dove and Ann Hochschild
Using the Yeast Two-Hybrid System to Identify Interacting Proteins
John Miller and Igor Stagljar
Analysis of Protein–Protein Interactions Utilizing Dual Bait Yeast Two-Hybrid System
Ilya G. Serebriiskii and Elena Kotova
The Split-Ubiquitin Membrane-Based Yeast Two-Hybrid System
Safia Thaminy, John Miller, and Igor Stagljar
Reverse Two-Hybrid Techniques in the Yeast Saccharomyces cerevisiae
Matthew A. Bennett, Jack F. Shern, and Richard A. Kahn
Mammalian Two-Hybrid Assay for Detecting Protein–Protein Interactions In Vivo
Jae Woon Lee and Soo-Kyung Lee
Co-Immunoprecipitation from Transfected Cells
Shane C. Masters
Part IV: Probing Protein–Protein Interactions in Living Cells
Microscopic Analysis of Fluorescence Resonance Energy Transfer (FRET)
Brian Herman, R. V. Krishnan, and Victoria E. Centonze
Monitoring Molecular Interactions in Living Cells Using Flow Cytometric Analysis of Fluorescence Resonance Energy Transfer
Francis Ka-Ming Chan
Fluorescence Correlation Spectroscopy: A New Tool for Quantification of Molecular Interactions
Keith M. Berland
Confocal Microscopy for Intracellular Co-localization of Proteins
Toshiyuki Miyundefined*a~Kbr_~H~M~2~1~Kbr_~H~M~2~1Mapping_Biochemical_Networks_with_Protein~FFragment_Complementation_Assays~Kbr_~H~M~2~1Ingrid_Remy_and_Stephen_W._Michnick~Kbr_~H~M~2~1~Kbr_~H~M~2~1In_Vivo_Protein_Cross~FLinking~Kbr_~H~M~2~1Fabrice_Agou~E_Fei_Ye~E_and_Michel_Véron
Part V. Proteomics-Based Approaches
Computational Prediction of Protein–Protein Interactions
John C. Obenauer and Michael B. Yaffe
Affinity Methods for Phosphorylation-Dependent Interactions
Greg Moorhead and Carol MacKintosh
Two-Dimensional Gel Electrophoresis for Analysis of Protein Complexes
Karin Barnouin
Sample Preparation of Gel Electrophoretically Separated Protein Binding Partners for Analysis by Mass Spectrometry
Rainer Cramer, Malcolm Saxton, and Karin Barnouin
Quantitative Protein Analysis by Solid Phase Isotope Tagging and Mass Spectrometry
Huilin Zhou, Rosemary Boyle, and Ruedi Aebersold
Internet Resources for Studying Protein–Protein Interactions
Shane C. Masters
Index
真希望有看到的一天