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Leukocyte Transmigration Through Vascular Endothelium: An In Vitro Method

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The endothelial lining of blood vessels forms an intact monolayer throughout the vascular system, and constitutes a critical barrier between the blood flow and the tissues beneath. Endothelial cells (EC) have a prominent role in the regulation of hemostasis, immunity, and inflammation; in particular, EC are active participants in regulating the traffic of cells and substances from blood to tissues and vice-versa (1 ,2 ).
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