Coning Hybridoma cDNA by RACE

互联网2014-02-13

1022

Hybridoma cDNA can be difficult to clone using V region PCR. This is due to mutations in primer regions as well as the presence of other expressed V genes in the hybridoma. In this chapter, the use of RACE to clone V genes is described. This avoids some of the problems associated with the use of V gene PCR.

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