Recombination and Site-Directed Mutagenesis Using Recombination PCR
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The polymerase chain reaction (PCR) (1 ) provides a rapid means for the recombination and site-directed mutagenesis of DNA (2 ). DNA modification can occur during PCR because the primers are incorporated into the ends of the PCR product. The simplest PCR-based method for site-directed mutagenesis and DNA recombination is recombination PCR.
