CELLULAR ELECTRON MICROSCOPY

Description

Recent advances in the imaging technique electron microscopy (EM)have improved the method,making it more reliable and rewarding,particularly in its description of three-dimensional detail。This book will help biologists from many disciplines understand modern EM and the value it might bring to their own work。The book?s five sections deal with all major issues in EM of cells: specimen preparation,imaging in 3-D,imaging and understanding frozen-hydrated samples,labeling macromolecules,and analyzing EM data。Each chapter was written by scientists who are among the best in their field,and some chapters provide multiple points of view on the issues they discuss。Each section of the book is preceded by an introduction,which should help newcomers understand the subject。The book shows why many biologists believe that modern EM will forge the link between light microscopy of live cells and atomic resolution studies of isolated macromolecules,helping us toward the goal of an atomic resolution understanding of living systems.

全书分4部分

1.Preparing Cells for Electron Microscopy
　　2.Imaging Fixed Cells in Three Dimensions
　　3.Imaging Frozen‐Hydrated Cells and Cell Parts
　　4.Localizing Macromolecules in Cells
　　5.Aspects of Data Collection and Analysis

Introduction: McIntosh - A new era in electron microscopy of cells

Preparative Methods for Cellular Electron Microscopy

1.The physics of rapid cooling and its implications for cryo-immobilization of cells
　　2.Cryopreparation Methods for Electron Microscopy of Selected Model Systems
　　3.Cryo-preparation methodology for plant cell biology
　　4.Correlative Light and Electron Microscopy of Early C。elegans Embryos in Mitosis

Imaging Fixed Cells in Three Dimensions

5.Understanding microtubule organizing centers by comparing mutant and wild-type structures with electron tomography
　　6.Whole Cell Investigation of Microtubule Cytoskeleton Architecture by Electron Tomography
　　7.Electron Microscopy of archaea
　　8.Reconstructing Mammalian Membrane Architecture by Large Area Cellular Tomography
　　9.Visualization of Membrane-Cytoskeletal Interactions during Plant Cytokinesis
　　10.Electron Tomographic Methods for Studying the Chemical Synapse
　　11.Using electron microscopy to understand functional mechanisms of chromosome alignment on the mitotic spindle
　　12.EM Analysis of the Leading Edge in Migrating Cells
　　13.Imaging actomyosin in situ

Imaging Frozen-Hydrated Cells and Cell Parts

14.Electron tomography of bacterial chemotaxis receptor assemblies
　　15.How to “read” a vitreous section
　　16.Single Particle Electron Cryomicroscopy of the Ion Channels in the Excitation-Contraction Coupling Junction
　　17.Electron microscopy of microtubule-based cytoskeletal machinery
　　18.Reconstructing the endocytotic machinery

Localizing Macromolecules in Cells

19.3-D Immuno-localization with plastic sections
　　20.EM analysis of viral morphogenesis
　　21.Electron tomography of immuno-labeled cryosections
　　22.Visualizing macromolecules with fluoronanogold: from photon microscopy to electron tomography
　　23.Markers for correlated light and electron microscopy
　　24.Localizing Specific Elements Bound to Macromolecules by EFTEM
　　25.Localization of protein complexes by pattern recognition

Aspects of Data Collection and Analysis Instrumentation for image acquisition

26.Application of Energy Filtered Electron Microscopy to Tomography of Thick,Selectively Stained Biological Samples
　　27.Optimization of Image Collection for Cellular Electron Microscopy
　　28.Future Directions for Camera Systems in Electron Microscopy

Computational methods for the analysis of cellular data

29.Structure determination in situ by averaging of tomograms
　　30.Methods for image segmentation in cellular tomography
　　31.Database Resources for Cellular Electron Microscopy