RNA电泳
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・ RNA Gel (Crawford Lab)
・ Gel Electrophoresis of RNA (Beverly Faulkner-Jones)
great tips on RNA gel electrophoresis.
・ Northern Gel and Transfer (gopher://iubio.bio.indiana.edu)
Using glyoxalated RNA
・ RNA Electrophoresis (Molecular Genetics RecLab)
・ RNA Electrophoresis in Denaturing Formaldehyde Gel (Molecular Genetics Rec Lab)
・ Formaldehyde-Agarose Gels (Beverly Faulkner-Jones)
Agarose gels have a smaller resolving power than polyacrylamide gels but a greater range of separation - from 200 bp to >50 kb using standard gels and electrophoresis equipment. RNAs up to 10 000 kb can be separated in agarose gels using pulsed field gel electrophoresis.
・ Denaturing polyacrylamide gel electrophoresis of RNA (Beverly Faulkner-Jones)
Polyacrylamide gels have enough resolving power to separate fragments differing by only one base pair in size, but their range is ~ 5 to 1000 bp. They are much more difficult to handle than agarose gels.
・ Recovery of RNA fragments from polyacrylamide gels (Beverly Faulkner-Jones)
The best method is the crush and soak method. It can be sued to isolate single-stranded RNA from denaturing gels. The RNA is of high purity but the method is long and inefficient if long transcripts are to be recovered (cf. less than 30% of DNA greater than 3 kb is recovered).
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Polyacrylamide Gel Electrophoresis (PAGE) of RNA (Frank Bottone)
For use withRibonuclease Protection Assay (RPA):