丁香实验_LOGO
登录
提问
我要登录
|免费注册
点赞
收藏
wx-share
分享

Intracellular Calcium Assays in Dissociated Primary Cortical Neurons

互联网

526
In this chapter, the basic tools and methods to isolate neurons from the embryonic rat cortex are provided. We outline the isolation of fresh neuronal cells and their storage, post-thaw maintenance, and the application of techniques to measure intracellular calcium changes in response to the addition of neurotransmitters. Many of these techniques come in kit formats supplied by companies like Invitrogen and thus enhance the ease of use, time required, and reproducibility.
提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
扫码领资料
反馈
TOP
打开小程序