Aggregation Effect in Microarray Data Analysis

Inferring gene regulatory networks from microarray data has become a popular activity in recent years, resulting in an ever-increasing volume of publications. There are many pitfalls in network analysis that remain either unnoticed or scantily understood. A critical discussion of such pitfalls is long overdue. Here we discuss one feature of microarray data the investigators need to be aware of when embarking on a study of putative associations between elements of networks and pathways.