Mascot检索的问题
丁香园论坛
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各位大侠，有个问题比较困惑，在Mascot上检索出来的蛋白并不是一一对应的，而是给出了几类蛋白，那么该如何选择呢？另外我的样本来自小鼠，如果检出的蛋白是（Rattus)的，是不是就不对呢？下面是一个例子，请各位百忙之中忙给予答复，谢谢。
1. gi|27691060 Mass: 16225 Score: 67 Expect: 0.025 Queries matched: 7
similar to tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide; protein kinase C inhibitor protein-1; phospholipase A2; 14-3-3 zeta [Ra
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2. gi|200915 Mass: 10311 Score: 51 Expect: 0.88 Queries matched: 4
serum amyloid A (AA at 131)
gi|6755394 Mass: 13613 Score: 47 Expect: 2.3 Queries matched: 4
serum amyloid A 2 [Mus musculus]
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3. gi|34862239 Mass: 14818 Score: 48 Expect: 1.8 Queries matched: 5
hypothetical protein XP_347080 [Rattus norvegicus]
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4. gi|38085824 Mass: 65935 Score: 47 Expect: 2.4 Queries matched: 7
similar to KRAB zinc finger protein KR18 [Mus musculus]
gi|28486463 Mass: 16720 Score: 32 Expect: 67 Queries matched: 3
similar to brain otoferlin [Mus musculus]
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5. gi|34873635 Mass: 53305 Score: 43 Expect: 6.2 Queries matched: 5
similar to hypothetical protein FLJ22625 [Rattus norvegicus]
1. gi|27691060 Mass: 16225 Score: 67 Expect: 0.025 Queries matched: 7
similar to tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide; protein kinase C inhibitor protein-1; phospholipase A2; 14-3-3 zeta [Ra
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2. gi|200915 Mass: 10311 Score: 51 Expect: 0.88 Queries matched: 4
serum amyloid A (AA at 131)
gi|6755394 Mass: 13613 Score: 47 Expect: 2.3 Queries matched: 4
serum amyloid A 2 [Mus musculus]
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3. gi|34862239 Mass: 14818 Score: 48 Expect: 1.8 Queries matched: 5
hypothetical protein XP_347080 [Rattus norvegicus]
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4. gi|38085824 Mass: 65935 Score: 47 Expect: 2.4 Queries matched: 7
similar to KRAB zinc finger protein KR18 [Mus musculus]
gi|28486463 Mass: 16720 Score: 32 Expect: 67 Queries matched: 3
similar to brain otoferlin [Mus musculus]
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5. gi|34873635 Mass: 53305 Score: 43 Expect: 6.2 Queries matched: 5
similar to hypothetical protein FLJ22625 [Rattus norvegicus]
