Analyzing PPAR/Ligand Interactions by Chemical Cross-Linking and High-Resolution Mass Spectrometry
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The combination of chemical cross-linking and high-resolution mass spectrometry is an emerging technique for monitoring conformational
changes in proteins induced by drug binding. In this chapter, we describe this approach for gaining insights into the conformational
changes of the peroxisome proliferator-activated receptor alpha after binding of low-molecular weight ligands. Our strategy
provides a basis to efficiently characterize target protein-drug interactions.
