Cleaning worms by floating on sucrose
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Cleaning worms by floating on sucrose
Have cold 70% sucrose in water.Wash worms off plates, put on ice.
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Typically we use 2 - 10 10-cm plates of crowded or starved worms
For this many worms, use a 15ml orange-cap centrifuge tube
Wash off with M9, S-basal, or water
Be sure to grow worms on agarose plates when making DNA to be cut
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This is optional, it makes them more buoyant
For a 15ml tube, spin in clinical centrifuge at #3 or 4
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30 - 35% sucrose final, 7 - 10 ml total
Keep cold, worms will thrash
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for a 15ml tube, spin in clinical centrifuge at #6 or 7
Draw off floating worms with a pasteur pipette.
Wash worms free of any sucrose.
If worms are to be kept alive, be very careful to use aseptic technique
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Bacteria, dead worms and other dense things will be on the bottom