YAC Library Screening II: Hybridization and PCR-Based Screening Protocols
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Yeast artificial chromosome (YAC) libraries stored in microtiter plates are available for screening as either complex PCR pools or hybridization filters generated from YACs gridded at high densities (see Chapter 3 ). Different libraries may be available as either PCR pools, hybridization filters, or both. Consequently, screening strategies have been designed that rely solely on either technique, or use a combined approach. Clearly, access to the YAC library microtiter plates and an automatic gridding system allows the user greater flexibility and is an advantage in large mapping projects.