丁香实验_LOGO
登录
提问
我要登录
|免费注册
点赞
收藏
wx-share
分享

An Overview of High-Throughput Screening Systems for Enantioselective Enzymatic Transformations

互联网

330
The directed evolution of enantioselective enzymes (1 ,2 ) for use in organic synthesis constitutes an attractive alternative to traditional forms of asymmetric catalysis based on chiral transition metal complexes or catalytic antibodies. It involves the proper combination of molecular biological methods for random gene mutagenesis and gene expression coupled with appropriate high-throughput screening systems that allow the rapid determination of the enantiomeric purity of a chiral product. Typically, thousands of samples arising from the catalytic action of the evolved enzyme variants on a given substrate of interest need to be assayed within a reasonable time span, ideally within one day.
提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
扫码领资料
反馈
TOP
打开小程序