High Resolution PCR Quantitation by AmpliSensor Assay
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A real-time kinetic tool for polymerase chain reaction (PCR) quantitation, AmpliSensor assay (1 ) quantifies PCR product by relating the rate of an amplification reaction through the progressive depletion of a rate-limiting primer. AmpliSensor assay invokes a two-step amplification scheme:
| 1. |
An initial amplification with one primer in excess in order to overproduce one strand of the target, and
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| 2. |
A subsequent hemi-nested amplification for fluorescence signal detection.
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