Neuropeptide RNA Localization in Tissue Sections
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In situ hybridization has become a routine technique to provide insights into RNA localization. However, different protocols
exist for multiple purposes, and it is, therefore, important to clearly define specific needs to choose the most suitable
one(s). For instance, in situ hybridization can target different types of RNA, including mRNA or small noncoding RNA such
as micro RNA (miRNA). Detection protocols are developed for light or electron microscopy and can be combined with immunocytochemistry
to study RNA coexpression with proteins or peptides. In this chapter, we present some protocols to illustrate the diversity
of in situ hybridization methods. We focus on the detection of mRNA or miRNA and show that the protocols are quite similar
but use dedicated probe types, namely, oligo- or riboprobes and locked nucleic-acid probes.