COLONY HYBRIDIZATION
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COLONY HYBRIDIZATION
1) CUT 4 PIECES OF 3MM WHATMAN PAPER AND PLACE EACH ONE IN A SEPARATE CONTAINER(A CAFETERIA TRAY WILL PROBABLY WORK PERFECTLY).
2) SATURATE EACH OF THE 4 PIECES WITH ONE OF THE FOLLOWING SOLUTIONS:
A) 10% SDS
B) Denaturing Solution (.5N NaOH, 1.5M NaCl)
C) Neutralizing solution (1.5M NaCl,.5M Tris pH 7.4)
D) 2X SSC