Interaction of the Glycocalyx with the Actin Cytoskeleton

In this chapter, we summarize and describe various methods that have been used to detect glycocalyx and to study the interactions between glycocalyx and actin cytoskeleton with the emphasis in the nervous tissue. We highlight two methodologies to analyze the direct interaction between glycocalyx and actin cytoskeleton: one method focusing on visualization using immunostaining and confocal microscopy techniques and the other focusing on direct protein–protein interactions using co-immunoprecipitation techniques.