Binding Studies with Flow Cytometry

Flow cytometry allows the multiparametric physical and biochemical analysis of single cells within large cell suspensions which is most frequently for cell counting and for the measurement of antigen expression with fluorescence conjugated antibodies. Moreover, flow cytometry also represents one of the most crucial test systems for the development of therapeutic antibodies because antibodies have to bind their antigen in its natural context with relatively high affinity. The following two protocols describe the extracellular and intracellular staining of cells with recombinant antibodies and antibody fusion proteins.