丁香实验_LOGO
登录
提问
我要登录
|免费注册
点赞
收藏
wx-share
分享

Practical Considerations for Collecting Confocal Images

互联网

690
Conventional microscopy delivers two-dimensional images in real time and real color to the eye of the user. Confocal microscopy adds a third dimension by imaging only one plane within the sample at a time so that variations in depth can be quantified (1 ). This has both positive and negative aspects. The advantage is that a series of such slices can be reconstructed to give 3D views and enable volume analysis of the sample, and that any one slice is crisper and clearer than a full-field fluorescence image. The disadvantage is that the portion of the sample visible at any one time is so small that finding the most interesting parts of the specimen may no longer be possible (Fig. 1 ).
http://img.dxycdn.com/trademd/upload/asset/meeting/2014/02/13/B1392271807.jpg
Fig. 1.  Specimen space vs sampled volume in a confocal and a bright field microscope.

提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
扫码领资料
反馈
TOP
打开小程序