引物评分!急!!
丁香园论坛
2432
我做的是人巨细胞病毒gB基因,序列如下
ccgcggccgtctcgggtgtcttcagggagccgcaccgaccttggctgccaagtccgtatc
cctcctcctcgactgcgggtgtttcccggagggtccgcgcgacacgcaagagaccacgac
gcgcctcatcgctgctggatttggcccgcgacgaacatggaatccaggatctggtgcctg
gtagtctgcgttaacctgtgtatcgtctgtctgggtgctgcggtttcctcttctagtact
tcccatgcaacttcttctactcacaatggaagccatacttctcgtacgacgtctgctcaa
acccggtcagtctattctcaacacgtaacgtcttctgaagccgtcagtcatagagccaac
gagactatctacaacactaccctcaagtacggagatgtggtgggagtcaacactaccaag
tacccctatcgcgtgtgttctatggcccagggtacggatcttattcgctttgaacgtaat
atcatctgcacctcgatgaagcctatcaatgaagacttggatgagggcatcatggtggtc
tacaagcgcaacatcgtggcgcacacctttaaggtacgggtctaccaaaaggttttgacg
tttcgtcgtagctacgcttacatctacaccacttatctgctgggcagcaatacggaatac
gtggcgcctcctatgtgggagattcatcacatcaacaagtttgctcaatgctacagttcc
tacagccgcgttataggaggcacggttttcgtggcatatcatagggacagttatgaaaac
aaaaccatgcaattaattcccgacgattattccaacacccacagtacccgttacgtgacg
gtcaaggatcagtggcacagccgcggcagcacctggctctatcgtgagacctgtaatctg
aactgtatgctgaccatcactactgcgcgctccaagtatccttatcatttttttgcaact
tccacgggtgatgtggtttacatttctcctttctacaacggaaccaatcgcaatgccagc
tactttggagaaaacgccgacaagtttttcattttcccgaactacaccatcgtttccgac
tttggaagacccaacgctgcgccagaaacccataggttggtggcttttctcgaacgtgcc
gactcggtgatctcttgggatatacaggacgagaagaatgtcacctgccagctcaccttc
tgggaagcctcggaacgtactatccgttccgaagccgaagactcgtaccacttttcttct
gccaaaatgactgcaacttttctgtctaagaaacaagaagtgaacatgtccgactccgcg
ctggactgcgtacgtgatgaggctataaataagttacagcagattttcaatacttcatac
aatcaaacatatgaaaaatacggaaacgtgtccgtcttcgaaaccagcggcggtctggtg
gtgttctggcaaggcatcaagcaaaaatctttggtggaattggaacgtttggccaatcga
tccagtctgaatatcactcataggaccagaagaagtacgagtgacaataatacaactcat
ttgtccagcatggaatcggtgcacaatctggtctacgcccagctgcagttcacctatgac
acgttgcgcggttacatcaaccgggcgctggcgcaaatcgcagaagcctggtgtgtggat
caacggcgcaccctagaggtcttcaaggaactcagcaagatcaacccgtcagccattctc
tcggccatttacaacaaaccgattgccgcgcgtttcatgggtgatgtcttgggcctggcc
agctgcgtgaccatcaaccaaaccagcgtcaaggtgctgcgtgatatgaacgtgaaggaa
tcgccaggacgctgctactcacgacccgtggtcatctttaatttcgccaacagctcgtac
gtgcagtacggtcaactgggcgaggacaacgaaatcctgttgggcaaccaccgcactgag
gaatgtcagcttcccagcctcaagatcttcatcgccgggaactcggcctacgagtacgtg
gactacctcttcaaacgcatgattgacctcagcagtatctccaccgtcgacagcatgatc
gccctggatatcgacccgctggaaaataccgacttcagggtactggaactttactcgcag
aaagagctgcgttccagcaacgtttttgacctcgaagagatcatgcgcgaattcaactcg
tacaagcagcgggtaaagtacgtggaggacaaggtagtcgacccgctaccgccctacctc
aagggtctggacgacctcatgagcggcctgggcgccgcgggaaaggccgttggcgtagcc
attggggccgtgggtggcgcggtggcctccgtggtcgaaggcgttgccaccttcctcaaa
aaccccttcggagccttcaccatcatcctcgtggccatagccgtagtcattatcacttat
ttgatctatactcgacagcggcgtctgtgcacgcagccgctgcagaacctctttccctat
ctggtgtccgccgacgggaccaccgtgacgtcgggcagcaccaaagacacgtcgttacag
gctccgccttcctacgaggaaagtgtttataattctggtcgcaaaggaccgggaccaccg
tcgtctgatgcatccacggcggctccgccttacaccaacgagcaggcttaccagatgctt
ctggccctggcccgtctggacgcagagcagcgagcgcagcagaacggtacagattctttg
gacggacagactggcacgcaggacaagggacagaagcctaacctgctagaccggctgcga
catcgcaaaaacggctacagacacttgaaagactccgacgaagaagagaacgtctgaacc
aggaggaaaaaaaaactagacaaaaaatattgacacagagacttgtgatatacggggtta
aactggatatctaggtgctgcatgtgtattttctttgtgattttgcttcgtaagctgtca
gcctctcacggtccgctatgtttttcaacccgtatctgagcggcggcgtgaccggcggtg
cggtcgcgggtggccggcgtcagcgttcgcagcccggctccgcgcagggctcgggcaagc
ggccg
现有一对引物,请各位帮忙评价一下:
p1:ggcatcaagcaaaaatct
p2: cagttgaccgtactgcac
多谢了,急!!
ccgcggccgtctcgggtgtcttcagggagccgcaccgaccttggctgccaagtccgtatc
cctcctcctcgactgcgggtgtttcccggagggtccgcgcgacacgcaagagaccacgac
gcgcctcatcgctgctggatttggcccgcgacgaacatggaatccaggatctggtgcctg
gtagtctgcgttaacctgtgtatcgtctgtctgggtgctgcggtttcctcttctagtact
tcccatgcaacttcttctactcacaatggaagccatacttctcgtacgacgtctgctcaa
acccggtcagtctattctcaacacgtaacgtcttctgaagccgtcagtcatagagccaac
gagactatctacaacactaccctcaagtacggagatgtggtgggagtcaacactaccaag
tacccctatcgcgtgtgttctatggcccagggtacggatcttattcgctttgaacgtaat
atcatctgcacctcgatgaagcctatcaatgaagacttggatgagggcatcatggtggtc
tacaagcgcaacatcgtggcgcacacctttaaggtacgggtctaccaaaaggttttgacg
tttcgtcgtagctacgcttacatctacaccacttatctgctgggcagcaatacggaatac
gtggcgcctcctatgtgggagattcatcacatcaacaagtttgctcaatgctacagttcc
tacagccgcgttataggaggcacggttttcgtggcatatcatagggacagttatgaaaac
aaaaccatgcaattaattcccgacgattattccaacacccacagtacccgttacgtgacg
gtcaaggatcagtggcacagccgcggcagcacctggctctatcgtgagacctgtaatctg
aactgtatgctgaccatcactactgcgcgctccaagtatccttatcatttttttgcaact
tccacgggtgatgtggtttacatttctcctttctacaacggaaccaatcgcaatgccagc
tactttggagaaaacgccgacaagtttttcattttcccgaactacaccatcgtttccgac
tttggaagacccaacgctgcgccagaaacccataggttggtggcttttctcgaacgtgcc
gactcggtgatctcttgggatatacaggacgagaagaatgtcacctgccagctcaccttc
tgggaagcctcggaacgtactatccgttccgaagccgaagactcgtaccacttttcttct
gccaaaatgactgcaacttttctgtctaagaaacaagaagtgaacatgtccgactccgcg
ctggactgcgtacgtgatgaggctataaataagttacagcagattttcaatacttcatac
aatcaaacatatgaaaaatacggaaacgtgtccgtcttcgaaaccagcggcggtctggtg
gtgttctggcaaggcatcaagcaaaaatctttggtggaattggaacgtttggccaatcga
tccagtctgaatatcactcataggaccagaagaagtacgagtgacaataatacaactcat
ttgtccagcatggaatcggtgcacaatctggtctacgcccagctgcagttcacctatgac
acgttgcgcggttacatcaaccgggcgctggcgcaaatcgcagaagcctggtgtgtggat
caacggcgcaccctagaggtcttcaaggaactcagcaagatcaacccgtcagccattctc
tcggccatttacaacaaaccgattgccgcgcgtttcatgggtgatgtcttgggcctggcc
agctgcgtgaccatcaaccaaaccagcgtcaaggtgctgcgtgatatgaacgtgaaggaa
tcgccaggacgctgctactcacgacccgtggtcatctttaatttcgccaacagctcgtac
gtgcagtacggtcaactgggcgaggacaacgaaatcctgttgggcaaccaccgcactgag
gaatgtcagcttcccagcctcaagatcttcatcgccgggaactcggcctacgagtacgtg
gactacctcttcaaacgcatgattgacctcagcagtatctccaccgtcgacagcatgatc
gccctggatatcgacccgctggaaaataccgacttcagggtactggaactttactcgcag
aaagagctgcgttccagcaacgtttttgacctcgaagagatcatgcgcgaattcaactcg
tacaagcagcgggtaaagtacgtggaggacaaggtagtcgacccgctaccgccctacctc
aagggtctggacgacctcatgagcggcctgggcgccgcgggaaaggccgttggcgtagcc
attggggccgtgggtggcgcggtggcctccgtggtcgaaggcgttgccaccttcctcaaa
aaccccttcggagccttcaccatcatcctcgtggccatagccgtagtcattatcacttat
ttgatctatactcgacagcggcgtctgtgcacgcagccgctgcagaacctctttccctat
ctggtgtccgccgacgggaccaccgtgacgtcgggcagcaccaaagacacgtcgttacag
gctccgccttcctacgaggaaagtgtttataattctggtcgcaaaggaccgggaccaccg
tcgtctgatgcatccacggcggctccgccttacaccaacgagcaggcttaccagatgctt
ctggccctggcccgtctggacgcagagcagcgagcgcagcagaacggtacagattctttg
gacggacagactggcacgcaggacaagggacagaagcctaacctgctagaccggctgcga
catcgcaaaaacggctacagacacttgaaagactccgacgaagaagagaacgtctgaacc
aggaggaaaaaaaaactagacaaaaaatattgacacagagacttgtgatatacggggtta
aactggatatctaggtgctgcatgtgtattttctttgtgattttgcttcgtaagctgtca
gcctctcacggtccgctatgtttttcaacccgtatctgagcggcggcgtgaccggcggtg
cggtcgcgggtggccggcgtcagcgttcgcagcccggctccgcgcagggctcgggcaagc
ggccg
现有一对引物,请各位帮忙评价一下:
p1:ggcatcaagcaaaaatct
p2: cagttgaccgtactgcac
多谢了,急!!