A Novel Immobilization Method for Entrapment: LentiKats
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Immobilization by entrapment in hydrogel particles based on polyvinyl alcohol (PVA) shows many advantages in immobilizing living cells and cross-linked enzymes. The immobilization takes place at room temperature in aqueous environment. The outstand-ing advantages compared to other immobilization methods are described. Subsequently the different possibilities for forming these hydrogel particles are introduced and many practical tips are given for understanding important parameters like initial biomass content or the survival rate during the immobilization step.
The benefit of this immobilization technique is shown by some examples of successful immobilization of whole cells and enzymes in LentiKats� : bioconversion of raw glycerol to 1,3-propanediol, bioethanol production from nonsterile molasses, reduction of energy consumption for sewage treatment, and production of (R)-cyanohydrins by using entrapped (R)-oxynitrilase in LentiKats.
