Oligonucleotide purification

1. Add 400 μl of TE buffer then 400 μl of 1-butanol to the oligonucleotide glass vial.

2. Vortex well, then spin down in tabletop centrifuge for 10 minutes at ~2,000. rpm¹s.

3. Remove top, butanol layer with a sterile pipette tip and discard.

4. Add another 400 μl of 1-butanol.

5. Vortex well, then spin down as above in tabletop centrifuge.

6. Remove top, butanol layer and discard.

7. Transfer aqueous phase into a new 1.5 ml tube.

8. Dry in a speed vac for about 5 minutes to remove all of the butanol.

9. Add 50 μl of 5M KAc and fill tube with 95% ETOH.

10. Precipitate for at least one hour at -70℃.

11. Spin for 15 minutes/ 4℃/ maximum speed.

12. Pour off ETOH and dry in a speed vac as above.

13. Add 500 μl of TE buffer to your oligo. in the 1.5 ml tube and mix well.

14. Dilute 1:10 and/or 1:100 and read on spectrophotometer to determine concentration.

15. Follow product specification sheet to determine volume needed to make a 20 um solution.