【求助】友有没有什么能常温将RNA携带的方法
丁香园论坛
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请问战友有没有什么能常温将RNA携带的方法,求具体处理步骤,不胜感激
方法1: 利用INVITROGEN的RNALATER试剂保存RNA。
Room-temperature storage (18–25°C) in RNAlater is possible for up to 7 days according to manufacturer’s instructions, although significant mRNA degradation was detected after 3 days of room-temperature storage of rat liver samples soaked in RNAlater.
方法2: 用RNAstable来保存: http://www.mobitec.com/de/products/bio/02_genomics/index.php?RNAstable.html
方法3: 用GenVault来保存: http://www.nature.com/nmeth/journal/v5/n9/full/nmeth.f.219.html#abs
Room-temperature storage (18–25°C) in RNAlater is possible for up to 7 days according to manufacturer’s instructions, although significant mRNA degradation was detected after 3 days of room-temperature storage of rat liver samples soaked in RNAlater.
方法2: 用RNAstable来保存: http://www.mobitec.com/de/products/bio/02_genomics/index.php?RNAstable.html
方法3: 用GenVault来保存: http://www.nature.com/nmeth/journal/v5/n9/full/nmeth.f.219.html#abs
好像就放在异丙醇里面就可以,到了目的地再沉淀一下。
1. RNA 干粉保存
2.70%乙醇洗涤那步,不离心直接运输
2.70%乙醇洗涤那步,不离心直接运输
专业从事临床样本(细胞)门到门温控限时运输,省去研究者自行运输时遇到的较多麻烦,详细信息请参考:http://shanghai.baixing.com/kuaidi/a120175604.html
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