Slot-Blot Hybridization in Studying Gene Expression During Oxygen Deprivation

Gene expression plays an important role in many biological processes. During development, for example, early developmental genes are turned on first to guide embryonic pattern formation (1 –3 ). A stress, either a physiological/ pathophysiological or an environmental one, can also regulate the expression of certain genes. To meet the stressful challenges, an organism has to produce more chaperones or protective proteins (4 –6 ). A growing body of evidence shows that O₂ deprivation differentially regulates gene expression (7 –9 ).