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Other mycoplasma media

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1359

Base:

 Bacto Heart Infusion Broth  1.2%
 Bacto Yeast Extract 0.5%
Bacto Peptone 0.5%
NaCl 0.025%
Na-DNA 0.002%

pH is adjusted to 8 and autoclaved.

May be stored at room temperature for 4 weeks

ADD (to final conc.)

 K2HPO4 0.17%
Glucose 1.0%
Thallium Acetate 0.025%
Penicillin 500u/ml
Horse Serum 10%
Na-DNA 0.0025%

pH to 7.8

4% culture is added and grown statically at 37°C for 18-20 hours to A260

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