Optimization of Sensitivity
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Action to improved sensivity |
Drawback(s) to consider |
---|---|
Employ low-UV wavelength |
Increased background noise—determine wavelength to give optimum signal-to-noise ratio |
Increase capillary bore |
Increased current, reduced EOF giving possible alteration in selectivity |
Increased injection time |
Reduction in separation efficiency; excessive time will result in run failure |
Appropriate use of electrokinetic injection |
Sampling bias for more mobile ions, sample matrix effects on injection amount |
Increased electrolyte strength |
Increased current and associated noise |
Optimize electrolyte composition |
Effects on selectivity and current |
Decrease operating voltage |
Increase in analysis time |
Decrease temperature |
Increase in analysis time |
Capillary modifications |
Reduction in separation efficiency and resolution, increased cost |
Sample derivatization |
Additional sampling handling |
Indirect detection |
Extra method development considerations |
Wide-bore capillaries |
EOF profile disturbed, adjustments to rinse and injection times, siphoning effects more pronounced |
Increased detector slit width |
Reduction in separation efficiency and resolution |