Isolation of Nucleic Acids
互联网
373
Thorough characterization of recombinant strains of Pichia pastoris requires analysis of genomic DNA and RNA. The protocols in this chapter describe methods for isolation of high-quality genomic DNA suitable for hybridization experiments, crude genomic DNA suitable for use as a PCR template, and total RNA suitable for a wide range of transcriptional analyses. They are based on standard protocols developed for Saccharomyces cerevisiae and have been modified by ourselves or others for P. pastoris .
