The DNA Alkaline Unwinding Genotoxicity Test

DNA is the target molecule for chemical and physical mutagens and carcinogens. These agents may attack DNA directly or modify other cel- lular processes associated with the integrity of the genome. The resulting alterations in the structure of DNA may either be irreversible, giving rise to mutations and cell death, or be repaired by various DNA repair enzymes without producing toxic effects. DNA damage can also be caused by agents that primarily affect the viability of the cell (1) .