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限制性内切酶添加保护碱基数目及酶切效率

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限制性核酸内切酶产品选择专题
&NBS p; 限制性内切酶 保护碱基A-B

&NBS p; 酶切效率:
&NBS p; - 0%
&NBS p; - 0-20%
- 20-50%
- 50-100%
~undefined - 反应时间为16小时

保护碱基数目
1 2 3 4 5
AarI 20-50 50-100
AasI 50-100
AatII 0 0-20 20-50 50-100
Acc65I 0-20 50-100
AdeI 50-100
AluI 0 20-50 50-100
Alw21I 50-100
Alw26I 50-100
Alw44I 0 20-50 50-100
ApaI 50-100
BamHI 50-100
BcnI 20-50 50-100
BclI 0 50-100
BcuI 50-100
BfiI 50-100
BfmI 50-100
BfuI 50-100
BglI 20-50 50-100
BglII 0 50-100
Bme1390I 20-50 50-100
BoxI 0 50-100
BpiI 50-100
Bpu10I 20-50 50-100
Bpu1102I 50-100
BseDI 0 50-100
BseGI 50-100
BseJI 0 50-100
BseLI 0 50-100
BseMI 0-20 50-100
BseMII 50-100
BseNI 0 50-100
BseSI 50-100
BseXI 20-50 50-100
Bsh1236I 50-100
Bsh1285I 0-20 50-100
BshNI 50-100
BshTI 20-50 50-100
Bsp68I 0 50-100
Bsp119I 50-100
Bsp120I 20-50 50-100
Bsp143I 50-100
Bsp143II 0 50-100
Bsp1407I 20-50 50-100
BspLI 50-100
BspPI 0 50-100
BspTI 0 0-20 50-100
Bst1107I 0-20 50-100
BstXI 0 50-100
Bsu15I 50-100
BsuRI 0-20 20-50 50-100

限制性内切酶保护碱基C-E

酶切效率 :
- 0%
- 0-20%
- 20-50%
- 50-100%
~undefined - 反应时间为16小时

保护碱基数目
1 2 3 4 5
CaiI 0 0-20 50-100
CfrI 0 50-100
Cfr9I 20-50 50-100
Cfr10I 20-50 50-100
Cfr13I 50-100
Cfr42I 50-100
CpoI 50-100
Csp6I 50-100
DpnI 50-100
DraI 0 0-20 50-100
Eam1104I 0 50-100
Eam1105I 0 50-100
Ecl136II 50-100
Eco24I 50-100
Eco31I 20-50 50-100
Eco32I 20-50 50-100
Eco47I 50-100
Eco47III 0 0-20 50-100
Eco52I 0-20 50-100
Eco57I 50-100
Eco57MI 50-100
Eco72I 0-20 50-100
Eco81I 50-100
Eco88I 50-100
Eco91I 20-50 50-100
Eco105I 20-50 50-100
Eco130I 0 50-100
Eco147I 0 50-100
EcoO109I 50-100
EcoRI 50-100
EherI 20-50 50-100
Esp3rI 50-100

限制性内切酶保护碱基F-N

酶切效率 :
- 0%
- 0-20%
- 20-50%
- 50-100%
~undefined - 反应时间为16小时

保护碱基数目
1 2 3 4 5
FaqI 0-20 50-100
FspAI 0-20 20-50 50-100
FspBI 20-50 50-100
GsuI 50-100
Hin1I 50-100
Hin1II 0 0-20 50-100
Hin6I 0 0-20 50-100
HincII 50-100
HindIII 0 0-20 50-100
HinfI 50-100
HpaII 0-20 50-100
HphI 0 50-100
Hpy8I 0-20 50-100
HpyF10VI 50-100
KpnI 50-100
Kpn2I 0 50-100
KspAI 20-50 50-100
LweI 20-50 50-100
MbiI 0 0-20 50-100
MboI 50-100
MboII 50-100
MlsI 0-20 50-100
MluI 20-50 50-100
MnlI 0 50-100
Mph1103I 20-50 50-100
MspI 0-20 50-100
MssI 20-50 50-100
MunI 20-50 50-100
MvaI 0 20-50 50-100
Mva1269I 0 50-100
NcoI 0 50-100
NdeI * 0-20 20-50 50-100
NheI 0 20-50 50-100
NmuCI 20-50 50-100
NotI 20-50 50-100
NsbI 0 0-20 50-100

限制性内切酶保护碱基0-X

酶切效率:
- 0%
- 0-20%
- 20-50%
- 50-100%
~undefined - 反应时间为16小时

保护碱基数目
1 2 3 4 5
OliI 0-20 20-50 50-100
PaeI 0 0-20 20-50 50-100
PagI 20-50 50-100
PauI 0 50-100
PdiI 0-20 50-100
PdmI 50-100
PfeI 50-100
Pfl23II 0-20 20-50 50-100
PfoI 0 20-50 50-100
Psp5II 0 50-100
Psp1406I 0-20 50-100
PsuI 0-20 50-100
PstI 0-20 50-100
PsyI 0 50-100
PvuI 20-50 50-100
PvuII 50-100
RsaI 50-100
SacI 50-100
SalI 20-50 50-100
SatI 0 50-100
ScaI 0-20 50-100
SchI 50-100
SdaI 0-20 50-100
SduI 50-100
SfiI 50-100
SmaI 50-100
SmiI 0-20 50-100
SmuI 50-100
SsiI 50-100
SspI 0-20 50-100
TaaI 20-50 50-100
TaiI 50-100
TaqI 0 20-50 50-100
TasI 0 20-50 50-100
TatI 0-20 20-50 50-100
TauI 20-50 50-100
Tru1I 0 0-20 50-100
Van91I 0 50-100
VspI 50-100
XagI 20-50 50-100
XapI 20-50 50-100
XbaI 20-50 50-100
XceI 0 50-100
XhoI 0-20 50-100
XmaJI 50-100
XmiI 50-100
I-SceI 50-100

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