&NBS p; 限制性内切酶 保护碱基A-B
&NBS p; |
酶切效率: |
&NBS p; |
- 0% |
&NBS p; |
- 0-20% |
|
- 20-50% |
|
- 50-100% |
~undefined |
- 反应时间为16小时 |
|
|
酶 |
保护碱基数目 |
1 |
2 |
3 |
4 |
5 |
AarI |
20-50 |
50-100 |
AasI |
50-100 |
AatII |
0 |
0-20 |
20-50 |
50-100 |
Acc65I |
0-20 |
50-100 |
AdeI |
50-100 |
AluI |
0 |
20-50 |
50-100 |
Alw21I |
50-100 |
Alw26I |
50-100 |
Alw44I |
0 |
20-50 |
50-100 |
ApaI |
50-100 |
BamHI |
50-100 |
BcnI |
20-50 |
50-100 |
BclI |
0 |
50-100 |
BcuI |
50-100 |
BfiI |
50-100 |
BfmI |
50-100 |
BfuI |
50-100 |
BglI |
20-50 |
50-100 |
BglII |
0 |
50-100 |
Bme1390I |
20-50 |
50-100 |
BoxI |
0 |
50-100 |
BpiI |
50-100 |
Bpu10I |
20-50 |
50-100 |
Bpu1102I |
50-100 |
BseDI |
0 |
50-100 |
BseGI |
50-100 |
BseJI |
0 |
50-100 |
BseLI |
0 |
50-100 |
BseMI |
0-20 |
50-100 |
BseMII |
50-100 |
BseNI |
0 |
50-100 |
BseSI |
50-100 |
BseXI |
20-50 |
50-100 |
Bsh1236I |
50-100 |
Bsh1285I |
0-20 |
50-100 |
BshNI |
50-100 |
BshTI |
20-50 |
50-100 |
Bsp68I |
0 |
50-100 |
Bsp119I |
50-100 |
Bsp120I |
20-50 |
50-100 |
Bsp143I |
50-100 |
Bsp143II |
0 |
50-100 |
Bsp1407I |
20-50 |
50-100 |
BspLI |
50-100 |
BspPI |
0 |
50-100 |
BspTI |
0 |
0-20 |
50-100 |
Bst1107I |
0-20 |
50-100 |
BstXI |
0 |
50-100 |
Bsu15I |
50-100 |
BsuRI |
0-20 |
20-50 |
50-100 |
|
限制性内切酶保护碱基C-E |
|
|
酶切效率 : |
|
- 0% |
|
- 0-20% |
|
- 20-50% |
|
- 50-100% |
~undefined |
- 反应时间为16小时 |
酶 |
保护碱基数目 |
1 |
2 |
3 |
4 |
5 |
CaiI |
0 |
0-20 |
50-100 |
CfrI |
0 |
50-100 |
Cfr9I |
20-50 |
50-100 |
Cfr10I |
20-50 |
50-100 |
Cfr13I |
50-100 |
Cfr42I |
50-100 |
CpoI |
50-100 |
Csp6I |
50-100 |
DpnI |
50-100 |
DraI |
0 |
0-20 |
50-100 |
Eam1104I |
0 |
50-100 |
Eam1105I |
0 |
50-100 |
Ecl136II |
50-100 |
Eco24I |
50-100 |
Eco31I |
20-50 |
50-100 |
Eco32I |
20-50 |
50-100 |
Eco47I |
50-100 |
Eco47III |
0 |
0-20 |
50-100 |
Eco52I |
0-20 |
50-100 |
Eco57I |
50-100 |
Eco57MI |
50-100 |
Eco72I |
0-20 |
50-100 |
Eco81I |
50-100 |
Eco88I |
50-100 |
Eco91I |
20-50 |
50-100 |
Eco105I |
20-50 |
50-100 |
Eco130I |
0 |
50-100 |
Eco147I |
0 |
50-100 |
EcoO109I |
50-100 |
EcoRI |
50-100 |
EherI |
20-50 |
50-100 |
Esp3rI |
50-100 |
|
限制性内切酶保护碱基F-N |
|
|
酶切效率 : |
|
- 0% |
|
- 0-20% |
|
- 20-50% |
|
- 50-100% |
~undefined |
- 反应时间为16小时 |
酶 |
保护碱基数目 |
1 |
2 |
3 |
4 |
5 |
FaqI |
0-20 |
50-100 |
FspAI |
0-20 |
20-50 |
50-100 |
FspBI |
20-50 |
50-100 |
GsuI |
50-100 |
Hin1I |
50-100 |
Hin1II |
0 |
0-20 |
50-100 |
Hin6I |
0 |
0-20 |
50-100 |
HincII |
50-100 |
HindIII |
0 |
0-20 |
50-100 |
HinfI |
50-100 |
HpaII |
0-20 |
50-100 |
HphI |
0 |
50-100 |
Hpy8I |
0-20 |
50-100 |
HpyF10VI |
50-100 |
KpnI |
50-100 |
Kpn2I |
0 |
50-100 |
KspAI |
20-50 |
50-100 |
LweI |
20-50 |
50-100 |
MbiI |
0 |
0-20 |
50-100 |
MboI |
50-100 |
MboII |
50-100 |
MlsI |
0-20 |
50-100 |
MluI |
20-50 |
50-100 |
MnlI |
0 |
50-100 |
Mph1103I |
20-50 |
50-100 |
MspI |
0-20 |
50-100 |
MssI |
20-50 |
50-100 |
MunI |
20-50 |
50-100 |
MvaI |
0 |
20-50 |
50-100 |
Mva1269I |
0 |
50-100 |
NcoI |
0 |
50-100 |
NdeI * |
0-20 |
20-50 |
50-100 |
NheI |
0 |
20-50 |
50-100 |
NmuCI |
20-50 |
50-100 |
NotI |
20-50 |
50-100 |
NsbI |
0 |
0-20 |
50-100 |
|
限制性内切酶保护碱基0-X |
|
|
酶切效率: |
|
- 0% |
|
- 0-20% |
|
- 20-50% |
|
- 50-100% |
~undefined |
- 反应时间为16小时 |
酶 |
保护碱基数目 |
1 |
2 |
3 |
4 |
5 |
OliI |
0-20 |
20-50 |
50-100 |
PaeI |
0 |
0-20 |
20-50 |
50-100 |
PagI |
20-50 |
50-100 |
PauI |
0 |
50-100 |
PdiI |
0-20 |
50-100 |
PdmI |
50-100 |
PfeI |
50-100 |
Pfl23II |
0-20 |
20-50 |
50-100 |
PfoI |
0 |
20-50 |
50-100 |
Psp5II |
0 |
50-100 |
Psp1406I |
0-20 |
50-100 |
PsuI |
0-20 |
50-100 |
PstI |
0-20 |
50-100 |
PsyI |
0 |
50-100 |
PvuI |
20-50 |
50-100 |
PvuII |
50-100 |
RsaI |
50-100 |
SacI |
50-100 |
SalI |
20-50 |
50-100 |
SatI |
0 |
50-100 |
ScaI |
0-20 |
50-100 |
SchI |
50-100 |
SdaI |
0-20 |
50-100 |
SduI |
50-100 |
SfiI |
50-100 |
SmaI |
50-100 |
SmiI |
0-20 |
50-100 |
SmuI |
50-100 |
SsiI |
50-100 |
SspI |
0-20 |
50-100 |
TaaI |
20-50 |
50-100 |
TaiI |
50-100 |
TaqI |
0 |
20-50 |
50-100 |
TasI |
0 |
20-50 |
50-100 |
TatI |
0-20 |
20-50 |
50-100 |
TauI |
20-50 |
50-100 |
Tru1I |
0 |
0-20 |
50-100 |
Van91I |
0 |
50-100 |
VspI |
50-100 |
XagI |
20-50 |
50-100 |
XapI |
20-50 |
50-100 |
XbaI |
20-50 |
50-100 |
XceI |
0 |
50-100 |
XhoI |
0-20 |
50-100 |
XmaJI |
50-100 |
XmiI |
50-100 |
I-SceI |
50-100 |
|