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Preparation and Transplantation of a Composite Graft of Epidermal Keratinocytes on Acellular Dermis

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Loss of skin because of burns or ulcers is a major medical problem, and is the impetus for the development of skin substitutes and skin replacement technologies. Efforts in this area have focused on developing suitable substitutes for the epidermis or for the dermis, as well as ways to combine both technologies in a composite skin. In this chapter, we describe the construction of a skin substitute of cultured human keratinocytes on acellular human dermis.
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