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Overview of Vector Design for Mammalian Gene Expression

互联网

447
The technology of expressing foreign genes in mammalian cells has become increasingly important to study a number of biological questions and as a primary method for production of proteins for pharmaceutical use. Mammalian cells are frequently used as a host for expression of foreign genes because:
1. 
DNA cloned from higher eukaryotic cells (both cDNAs and genomic clones) is readily expressed since the signals for transcription, mRNA processing, and translation are conserved in higher eukaryotic systems,
2. 
Proteins are expressed in a stable functional form since the machinery to facilitate proper protein folding and assembly are coserved in higher eukaryotic cells;
3. 
Many post-translational modifications, especially for those proteins that transit the secretory pathway, are efficiently performed, and;
4. 
Many proteins are readily secreted from mammalian cells providing the ability to isolate the protein from conditioned medium that contains low amounts of protein when cells are propagated under serum-free conditions.
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