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  • Protein Construct Expression and Purification Procedures  (Gimila's Lab)
      
  • Protein Expression (Mark's Lab)
      

         Purification of GST Fused Proteins (Contributed by Chia Jin Ngee )
Many people have vented out frustration over insoluble GST-fused proteins. This is a protocol for enzymatically active soluble GST-fused proteins.  All GST-fused proteins are rendered soluble with this technique though enzyme activitiy can range  from 30-90%.

         Bacculovirus Production : for protein expression (Mike A. Dyer)
This is a modified version of the pharmingen Baculogold kit

 

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