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PLATE Transformation

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<center> <u><font color="#990000">PLATE Transformation</font> </u></center>

 

 

<center> <font><font><i><font>"PLATE" is an acronym of the names of ingredients in the transformation solution: PEG, Lithium Acetate, Tris, and EDTA.</font> </i> </font></font></center>

 

-Use sterile technique and sterile solutions throughout this method.-

1. In a 15 ml culture tube, inoculate 2-3 ml of the appropriate media with a single colony of the yeast strain to be transformed. This is the starter culture.

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