Pheromone Halo Assay

 

<center> <p> <b><u><font color="#800000"><font>Pheromone Halo Assay</font> </font> </u> </b></p> </center>

 

REFERENCE: Hoffman, G., Garrison, T. R., and Dohlman, H. G., Analysis of RGS proteins in Saccharomyces cerevisiae , Methods Enzymol. 344:617-631, 2002.
 

-Use sterile technique and sterile solutions throughout this method.-

1. Grow a starter culture at 30 C with shaking (250 rpm) until it reaches saturation.

<center> <p> <font><font><img height="188" src="http://img.dxycdn.com/trademd/upload/asset/meeting/2013/09/05/A1378267614.gif" width="200" /> </font> </font></p> </center>