【共享】一些Protocols,希望对大家有用

无意中看到,觉得不错,拿来与大家分享,内容包括:
Useful resources:
Blocking with immunizing peptide protocol (BL)
Buffers and stock solutions
Fluorophore table
Troubleshooting tips

Immunohistochemistry (IHC) / Immunocytochemistry (ICC) protocols:
IHC-Fr staining protocol
IHC-P staining protocol
ICC (IF) protocol
Double immunofluorescence - sequential protocol
Double immunofluorescence - simultaneous protocol
Determining if antibody binds only phosphorylated protein
Troubleshooting tips

Chromatin Immunoprecipitation (ChIP) protocols:
N-ChIP protocol
X-ChIP protocol
ChIP Tips

Dot blot protocols:

Dot Blot protocol

ELISA protocols:
Direct ELISA protocol using primary antibody
Direct ELISA protocol using fluorescent substrate
Indirect ELISA protocol using primary and secondary antibodies
Sandwich ELISA protocol using a pair of primary antibodies

Flow cytometry / FACS protocols:

Direct flow cytometry (FACS) protocol
Indirect flow cytometry (FACS) protocol
Immunoprecipitation (IP) protocols:
Immunoprecipitation protocol

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