【共享】多本英文生物医学实验手册下载(PDF格式)
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这个地址里有很多蛋白纯化的手册,自己用E-MAIL注册一下!就可以下载了
http://www4.amershambiosciences.com/aptrix/upp00919.nsf/content/list_all_handbooks
2D Electrophoresis using immobilized pH gradients - Principles and Methods
Two-dimensional electrophoresis (2-D electrophoresis) is a powerful and widely used method for the analysis of complex protein mixtures extracted from cells, tissues, or other biological samples. ...
Affinity Chromatography, Principles & Methods
This handbook describes the role of affinity chromatography in the purification of biomolecules, the principle of the technique, the media available and how to select them, application examples and ...
Antibody Purification Handbook
The purpose of this handbook is to present the most effective and most frequently used strategies for sample preparation and purification of the many different forms of antibodies and antibody ...
ArrayVision Genomics Software : Rapid and automated analysis of genome arrays.
Array-based genetic analyses start with cDNAs or oligonucleotides, immobilized on a substrate. The array elements are hybridized with a single labeled sequence, or a labeled complex mixture derived ...
Automated silver and coomassie staining of polyacrylamide gels
DNA silver staining, 1 mm thick unbacked gels and 0.5 mm thick gels on plastic backing Silver staining is the most sensitive method for permanent staining of proteins or nucleic acids in ...
Automated staining of polyacrylamide gels with Hoefer Processor Plus
The new Processor Plus from Amersham Biosciences will help you get the jump on both Western blot processing and acrylamide gel staining. One intelligent fluid delivery system handles both ...
Biotrak assays: the complete guide
Amersham Biosciences is a leading supplier of bioassays for a wide range of research applications. These include cell signaling, tissue remodeling, metabolism/toxicology, and the study of cancer, ...
Blot processing with the Processor Plus
The new Hoefer Processor Plus from Amersham Biosciences will help you get the jump on both Western blot processing and acrylamide gel staining. One intelligent fluid delivery system handles both ...
Chromatofocusing with Polybuffer and PBE
The separation of proteins according to their isoelectric points by isoelectric focusing (IEF) in ampholytes has proven be a highly successful and popular technique. The resolution offered by ...
Expanded Bed Adsorption Handbook
This handbook introduces the principles of Expanded Bed Adsorption and serves as a practical guide to the use of STREAMLINE adsorbents and columns available from Amersham Biosciences. Critical ...
Ficoll-Paque Plus; For in vitro isolation of lymphocytes
Isolation of lymphocytes from whole human blood is often required in such clinical investigations as histocompatibility testing and the assay of cell mediated immune responses as well as in many ...
Fluorescence Imaging Principles and Methods
Fluorescent labelling and staining, when combined with an appropriate imaging instrument, is a sensitive and quantitative method that is widely used in molecular biology and biochemistry laboratories ...
Gel Filtration, Principles and Methods
Gel filtration is a robust technique that is well suited to handling biomolecules that are sensitive to changes in pH, concentration of metal ions or co-factors and harsh environmental conditions. ...
GST Gene Fusion System
This handbook describes the Glutathione S-transferase (GST) Gene Fusion System which is a versatile system for the expression, purification and detection of fusion proteins produced in Escherichia ...
Guide to isoelectric focusing
Isoelectric focusing methods are widely applied for the separation of proteins, peptides and enzymes. The principle: In a pH gradient the sample components migrate towards the anode or the cathode to ...
Hydrophobic Interaction Chromatography, Principles and Methods
This book can serve as an excellent introduction to the subject of HIC for those new to this method of separation. More experienced chromatographers can also benefit from the useful review. Topics ...
Ion Exchange Chromatography & Chromatofocusing, Principles and Methods
IEX for the separation of biomolecules was introduced in the 1960s and continues to play a major role in the separation and purification of biomolecules. Today, IEX is one of the most frequently used ...
Ion Exchange Chromatography, Principles and Methods
Adsorption chromatography depends upon interactions of different types between solute molecules and ligands immobilized on a chromatography matrix. The first type of interaction to be successfully ...
Microarray Handbook
Microarray analysis has emerged in the last few years as a flexible method for analyzing large numbers of nucleic acid fragments in parallel. Its origins can be traced to several different ...
Microcarrier cell culture - principles and methods
Cell culture techniques have become vital to the study of animal cell structure, function and differentiation and for the production of many important biological materials such as vaccines, enzymes, ...
Microcarrier Cell Culture: Scale-up Methods
Animal cell culture, vital to the study of structure, function, and differentiation, also provides important biologicals for the pharmaceutical industry, including viral vaccines, enzymes, hormones ...
PCR product analysis using semi-dry flatbed gel electrophoresis.
Polymerase chain reaction (PCR) has revolutionized the field of molecular biology due to its exceptional speed, specificity, and sensitivity as a tool for genetic analysis. Of note also is the ...
Percoll, Methodology and Applications
This manual provides the basic methodology for making and using gradients of Percoll. In addition, the Application Tables in the latter part of this manual provide numerous references for using ...
Protein Electrophoresis
This Technical Manual contains information that has been distilled from more than 30 years of laboratory experience in developing and verifying applications and product performance for protein ...
Protein Purification Handbook
The development of techniques and methods for protein purification has been an essential pre-requisite for many of the advancements made in biotechnology. The key to successful and efficient protein ...
Qualification and Performance Verification Logbook for Biochrom Ltd UV/Visible Spectrophotometers
The purpose of this manual is to assist users of Biochrom Ltd UV/Visible Spectrophotometers in the qualification of their instruments in accordance with the European Pharmacopoeia, Supplement 2000. ...
Radiochemical Decomposition - a guide to the properties and storage of radiolabelled compounds
Amersham Biosciences has more than fifty years of experience in the manufacture and supply of radiochemicals. A continuing programme of investigation of the self-decomposition of radiochemicals on ...
Reversed Phase Chromatography, Principles and Methods
This handbook is intended to serve as an introduction to the principles and applications of reversed phase chromatography of biomolecules and as a practical guide to the reversed phase ...
Review 23: Efficient Detection of Biomolecules by Autoradiography, Fluorography or Chemiluminescence, Part II
This review is intended to guide readers to the most appropriate exposure conditions for optimal sensitivity, resolution or quantitative accuracy when detecting radioisotopes by radiographic film or ...
Review 23: Efficient Detection of Biomolecules by Autoradiography, Fluorography or Chemiluminescence, Part I
This review is intended to guide readers to the most appropriate exposure conditions for optimal sensitivity, resolution or quantitative accuracy when detecting radioisotopes by radiographic film or ...
Safe and Secure - A Guide to working safely with radiolabelled compounds
Radiochemicals are widely use in a variety of scientific applications. Amersham Biosciences' many years of experience in the manufacture and use of radiolabelled compounds provides a valuable source ...
SPA Product Guide
The purpose of this document is to outline the full product offering of SPA Scintillation and SPA Imaging beads.
The Guide to Nucleic Acid Labelling & Detection
For many years the use of radiolabels was the method of choice for nucleic acid labelling and detection. More recently,however,considerable research has lead to the development of a comprehensive ...
The Guide to Nucleic Acid Labelling & Detection (pages 18-26)
Section from the Guide to Nucleic Acid Labelling & Detection , detailing radioactive labelling products. 18-1142-34
The Recombinant Protein Handbook: Protein Amplification and Simple Purification.
This handbook is intended for the general reader interested in the amplification and purification of recombinant proteins and for everyday use at the laboratory bench. The growth in the use of ...
http://www4.amershambiosciences.com/aptrix/upp00919.nsf/content/list_all_handbooks
2D Electrophoresis using immobilized pH gradients - Principles and Methods
Two-dimensional electrophoresis (2-D electrophoresis) is a powerful and widely used method for the analysis of complex protein mixtures extracted from cells, tissues, or other biological samples. ...
Affinity Chromatography, Principles & Methods
This handbook describes the role of affinity chromatography in the purification of biomolecules, the principle of the technique, the media available and how to select them, application examples and ...
Antibody Purification Handbook
The purpose of this handbook is to present the most effective and most frequently used strategies for sample preparation and purification of the many different forms of antibodies and antibody ...
ArrayVision Genomics Software : Rapid and automated analysis of genome arrays.
Array-based genetic analyses start with cDNAs or oligonucleotides, immobilized on a substrate. The array elements are hybridized with a single labeled sequence, or a labeled complex mixture derived ...
Automated silver and coomassie staining of polyacrylamide gels
DNA silver staining, 1 mm thick unbacked gels and 0.5 mm thick gels on plastic backing Silver staining is the most sensitive method for permanent staining of proteins or nucleic acids in ...
Automated staining of polyacrylamide gels with Hoefer Processor Plus
The new Processor Plus from Amersham Biosciences will help you get the jump on both Western blot processing and acrylamide gel staining. One intelligent fluid delivery system handles both ...
Biotrak assays: the complete guide
Amersham Biosciences is a leading supplier of bioassays for a wide range of research applications. These include cell signaling, tissue remodeling, metabolism/toxicology, and the study of cancer, ...
Blot processing with the Processor Plus
The new Hoefer Processor Plus from Amersham Biosciences will help you get the jump on both Western blot processing and acrylamide gel staining. One intelligent fluid delivery system handles both ...
Chromatofocusing with Polybuffer and PBE
The separation of proteins according to their isoelectric points by isoelectric focusing (IEF) in ampholytes has proven be a highly successful and popular technique. The resolution offered by ...
Expanded Bed Adsorption Handbook
This handbook introduces the principles of Expanded Bed Adsorption and serves as a practical guide to the use of STREAMLINE adsorbents and columns available from Amersham Biosciences. Critical ...
Ficoll-Paque Plus; For in vitro isolation of lymphocytes
Isolation of lymphocytes from whole human blood is often required in such clinical investigations as histocompatibility testing and the assay of cell mediated immune responses as well as in many ...
Fluorescence Imaging Principles and Methods
Fluorescent labelling and staining, when combined with an appropriate imaging instrument, is a sensitive and quantitative method that is widely used in molecular biology and biochemistry laboratories ...
Gel Filtration, Principles and Methods
Gel filtration is a robust technique that is well suited to handling biomolecules that are sensitive to changes in pH, concentration of metal ions or co-factors and harsh environmental conditions. ...
GST Gene Fusion System
This handbook describes the Glutathione S-transferase (GST) Gene Fusion System which is a versatile system for the expression, purification and detection of fusion proteins produced in Escherichia ...
Guide to isoelectric focusing
Isoelectric focusing methods are widely applied for the separation of proteins, peptides and enzymes. The principle: In a pH gradient the sample components migrate towards the anode or the cathode to ...
Hydrophobic Interaction Chromatography, Principles and Methods
This book can serve as an excellent introduction to the subject of HIC for those new to this method of separation. More experienced chromatographers can also benefit from the useful review. Topics ...
Ion Exchange Chromatography & Chromatofocusing, Principles and Methods
IEX for the separation of biomolecules was introduced in the 1960s and continues to play a major role in the separation and purification of biomolecules. Today, IEX is one of the most frequently used ...
Ion Exchange Chromatography, Principles and Methods
Adsorption chromatography depends upon interactions of different types between solute molecules and ligands immobilized on a chromatography matrix. The first type of interaction to be successfully ...
Microarray Handbook
Microarray analysis has emerged in the last few years as a flexible method for analyzing large numbers of nucleic acid fragments in parallel. Its origins can be traced to several different ...
Microcarrier cell culture - principles and methods
Cell culture techniques have become vital to the study of animal cell structure, function and differentiation and for the production of many important biological materials such as vaccines, enzymes, ...
Microcarrier Cell Culture: Scale-up Methods
Animal cell culture, vital to the study of structure, function, and differentiation, also provides important biologicals for the pharmaceutical industry, including viral vaccines, enzymes, hormones ...
PCR product analysis using semi-dry flatbed gel electrophoresis.
Polymerase chain reaction (PCR) has revolutionized the field of molecular biology due to its exceptional speed, specificity, and sensitivity as a tool for genetic analysis. Of note also is the ...
Percoll, Methodology and Applications
This manual provides the basic methodology for making and using gradients of Percoll. In addition, the Application Tables in the latter part of this manual provide numerous references for using ...
Protein Electrophoresis
This Technical Manual contains information that has been distilled from more than 30 years of laboratory experience in developing and verifying applications and product performance for protein ...
Protein Purification Handbook
The development of techniques and methods for protein purification has been an essential pre-requisite for many of the advancements made in biotechnology. The key to successful and efficient protein ...
Qualification and Performance Verification Logbook for Biochrom Ltd UV/Visible Spectrophotometers
The purpose of this manual is to assist users of Biochrom Ltd UV/Visible Spectrophotometers in the qualification of their instruments in accordance with the European Pharmacopoeia, Supplement 2000. ...
Radiochemical Decomposition - a guide to the properties and storage of radiolabelled compounds
Amersham Biosciences has more than fifty years of experience in the manufacture and supply of radiochemicals. A continuing programme of investigation of the self-decomposition of radiochemicals on ...
Reversed Phase Chromatography, Principles and Methods
This handbook is intended to serve as an introduction to the principles and applications of reversed phase chromatography of biomolecules and as a practical guide to the reversed phase ...
Review 23: Efficient Detection of Biomolecules by Autoradiography, Fluorography or Chemiluminescence, Part II
This review is intended to guide readers to the most appropriate exposure conditions for optimal sensitivity, resolution or quantitative accuracy when detecting radioisotopes by radiographic film or ...
Review 23: Efficient Detection of Biomolecules by Autoradiography, Fluorography or Chemiluminescence, Part I
This review is intended to guide readers to the most appropriate exposure conditions for optimal sensitivity, resolution or quantitative accuracy when detecting radioisotopes by radiographic film or ...
Safe and Secure - A Guide to working safely with radiolabelled compounds
Radiochemicals are widely use in a variety of scientific applications. Amersham Biosciences' many years of experience in the manufacture and use of radiolabelled compounds provides a valuable source ...
SPA Product Guide
The purpose of this document is to outline the full product offering of SPA Scintillation and SPA Imaging beads.
The Guide to Nucleic Acid Labelling & Detection
For many years the use of radiolabels was the method of choice for nucleic acid labelling and detection. More recently,however,considerable research has lead to the development of a comprehensive ...
The Guide to Nucleic Acid Labelling & Detection (pages 18-26)
Section from the Guide to Nucleic Acid Labelling & Detection , detailing radioactive labelling products. 18-1142-34
The Recombinant Protein Handbook: Protein Amplification and Simple Purification.
This handbook is intended for the general reader interested in the amplification and purification of recombinant proteins and for everyday use at the laboratory bench. The growth in the use of ...
