Components and Supplementation of Various Culture Media

Components and Supplementation of Various Culture Media


Abbreviations: DMEM = Dulbecco's Modified Eagle's Medium
FCS = Fetal Calf (or Bovine) Serum
GPS = Glutamine-Penicillin-Streptomycin
BME = b-Mercaptoethanol
DMSO = Dimethylsulfoxide
HEPES = [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid]
        Buffer
NEAA = Non-essential Amino Acids
N = Nucleosides


Feeder/STO Medium

DMEM
7% FCS
1X GPS


M15 (ES Cell Growth Medium)

DMEM
15% FCS
1X GPS
1X BME


2X Freezing Medium

This should be freshly prepared for each use.  Wear gloves when
working with DMSO.

60% DMEM
20% FCS
20% DMSO


Injection Medium

DMEM
5% FCS
20 mM HEPES pH 7.2-7.4

Embryo Culture Medium

DMEM
20% FCS
1X GPS
1X BME
1X NEAA
1X N

From the Laboratory of Dr. Allan Bradley              
Baylor College of Medicine, Houston, Texas