In Vitro Translation of the Angiotensin AT1 Receptor in Wheat-Germ Extracts

In vitro translation is a powerful method for specifically investigating mRNA translation independently of transcription, post-translational processing, and protein trafficking. There are several well-characterized in vitro translation systems including mammalian cell extracts (e.g., HeLa cells and Chinese Hamster Ovary cells) (1 ,2 ), rabbit reticulocyte lysates (3 ,4 ), Xenopus egg extracts (5 ), rye embryo (6 ), and wheat germ (7 ,8 ) that efficiently and faithfully translate heterologous mRNAs. Our laboratory is interested in investigating translational control of the angiotensin AT₁ receptor. Thus, we need a cell-free system that efficiently translates the angiotensin AT₁ receptor.